Molecular and Cellular Biology Core

核心C由主任和一名技术人员组成，他们将提供分子和细胞生物学 支持本计划项目建议书中的三个项目。这个核心将提供三个主要的 将协助每个实验室进行实验的功能；1)操纵 基因在体外和体内血管平滑肌细胞中的表达；2)生产和操作 重组蛋白应用于血管或细胞；3)从野生型或 基因敲除突变动物并在二维或三维细胞外培养 环境。核心导演在重组病毒载体的创建方面拥有相当丰富的经验 用于将基因输送到血管细胞以及siRNA技术来抑制原发细胞中的基因表达 血管细胞包括血管平滑肌细胞、周细胞和内皮细胞。此外，核心 在两个维度的原代血管细胞的培养和操作方面，Director拥有丰富的经验 或者是立体文化。这个核心还将提供一个重要的功能，以促进每个 研究人员将推进微血管内的基因表达技术，以便能够操纵 微循环功能。主任将参与与该提案有关的所有科学职能 能够恰当地服务于每个项目的需要，并促进每个研究的分子方面 方向。 这一核心将提供对整体功能和科学整合至关重要的服务 该计划项目拨款。所有项目都将依赖于该核心在每个 上述重点关注的三个主要领域。

Core C consists of the director as well as one technician who will provide molecular and cellular biology support for each of the three projects in this program project proposal. This core will provide three major functions which will be to assist each ofthe laboratories in experiments designed to; 1) manipulate gene expression within vascular smooth muscle cells in vitro and in vivo; 2) produce and manipulate recombinant proteins to be applied to vessels or cells; and 3) to isolate ceils from wild-type or knockout mutant animals and to culture them in two-dimensional or three-dimensional extracellular environments. The core director has considerable experience in the creation of recombinant viral vectors for gene delivery into vascular cells as well as siRNA technology to suppress gene expression in primary vascular cells including vascular smooth muscle cells, pericytes and endothelial cells. In addition, the core director has extensive experience in the culture and manipulation of primary vascular cells in either twodimensional or three-dimensional culture. This core will also provide an important function to facilitate each investigator to advance gene expression technologies within microvessels to be able to manipulate microcirculatory function. The director will participate in all scientific functions related to the proposal to be able to properly serve the needs of each project and to facilitate the molecular aspects of each research direction. This core will provide services that are essential for the overall function and scientific integration of the Program Project Grant. All projects will rely on the services provided by this core in each of the three main areas of focus described above.