Molecular and Cellular Biology Core

分子和细胞生物学核心

• 批准号: 7918622
• 负责人: George E Davis
• 金额: $ 13.7万
• 依托单位: UNIVERSITY OF MISSOURI-COLUMBIA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-03-22 至 2015-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7918622
• 关键词: Animals; Area; Blood Vessels; Cells; Endothelial Cells; Environment; Gene Delivery; Gene Expression; In Vitro; Knock-out; Laboratories; Molecular; Molecular and Cellular Biology; Pericytes; Program Research Project Grants; Recombinant Proteins; Recombinants; Research; Research Personnel; Services; Small Interfering RNA; Smooth Muscle Myocytes; Technology; Viral Vector; design; experience; extracellular; in vivo; mutant; programs; research study; two-dimensional

Core C consists of the director as well as one technician who will provide molecular and cellular biology support for each of the three projects in this program project proposal. This core will provide three major functions which will be to assist each ofthe laboratories in experiments designed to; 1) manipulate gene expression within vascular smooth muscle cells in vitro and in vivo; 2) produce and manipulate recombinant proteins to be applied to vessels or cells; and 3) to isolate ceils from wild-type or knockout mutant animals and to culture them in two-dimensional or three-dimensional extracellular environments. The core director has considerable experience in the creation of recombinant viral vectors for gene delivery into vascular cells as well as siRNA technology to suppress gene expression in primary vascular cells including vascular smooth muscle cells, pericytes and endothelial cells. In addition, the core director has extensive experience in the culture and manipulation of primary vascular cells in either twodimensional or three-dimensional culture. This core will also provide an important function to facilitate each investigator to advance gene expression technologies within microvessels to be able to manipulate microcirculatory function. The director will participate in all scientific functions related to the proposal to be able to properly serve the needs of each project and to facilitate the molecular aspects of each research direction. This core will provide services that are essential for the overall function and scientific integration of the Program Project Grant. All projects will rely on the services provided by this core in each of the three main areas of focus described above.

核心C由主管以及一名技术人员组成，他们将提供分子和细胞生物学 支持该计划项目建议中的三个项目中的每个项目。这个核心将提供三个主要 将为每个实验室提供旨在的实验的功能； 1）操纵 血管平滑肌细胞中的基因表达在体外和体内； 2）生产和操纵 重组蛋白要应用于血管或细胞； 3）将天花板与野生型或 敲除突变动物，并在二维或三维细胞外培养它们 环境。核心主任在创建重组病毒媒介方面具有丰富的经验 用于递送到血管细胞的基因以及siRNA技术以抑制原发性基因表达 血管细胞，包括血管平滑肌细胞，周细胞和内皮细胞。另外，核心 导演在两性二维中的原代血管细胞的培养和操纵方面都有丰富的经验 或三维文化。该核心还将提供一个重要功能，以促进每个 研究人员可以推进微血管内的基因表达技术，以便能够操纵 微循环功能。董事将参与与该提案有关的所有科学功能 能够正确满足每个项目的需求并促进每个研究的分子方面 方向。 该核心将提供对整体功能和科学整合至关重要的服务 计划项目赠款。所有项目都将依靠该核心在每个项目中提供的服务 上面描述的三个主要重点领域。