Animal Core

1.1简介 实验动物核心的主要目标是以更具成本效益和效率的方式将老鼠安置在所有三个拟议项目的中央设施中。转基因小鼠的产生已被证明是回答生物问题的一个非常强大的工具，特别是那些与人类疾病直接相关的问题。 然而，动物模型已经变得越来越复杂，需要考虑的变量包括小鼠品系、组织特异性启动子的选择以调节靶向基因表达，以及总体费用。 核心B的功能是作为一个集中资源来安置和维护动物，并将实验动物的总体成本保持在最低水平。此外。约翰·马尼斯博士将作为研究人员的科学资源，计划产生用于实验分析的具有多种基因类型的复合突变小鼠。表1列出了各种基因工程动物和正常对照小鼠品系，它们将用于该计划的科学研究，安置在核心设施中，并在本提案过程中使用。根据研究人员的需要，每个老鼠品系将被安置和产生成正比，并使用基于专门软件的方法(Granite)的繁殖和管理算法。

1.1 Introduction The principal goal of the Experimental Animal Core is to house mice in the more cost effective and efficient manner in a central facility for all three of the proposed projects. The generation of transgenic mice has proven to be a very powerful tool in answering biologic questions, especially those directly relevant to human disease. However, animal modeling has become increasingly complex with many variables to consider including mouse strain, the selection of tissue specific promoters to modulate targeted gene expression, as well as overall expense. The function of Core B is to serve as a centralized resource to house and maintain the animals and to keep the overall costs of experimental animals to a minimum for the program. In addition. Dr. John Manis will act as a scientific resource to the investigators in planning for the generation of compound mutant mice with multiple genotypes for experimental analysis. Table 1 lists the various genetically engineered animals and normal control mouse strains that will be employed in the scientific studies of the program, housed in the core facility, and used during the course of this proposal. Each mouse strain will be housed and generated in direct proportion to the need ofthe investigator, with a breeding and management algorithm using a specialized software based method (Granite).