Animal Core

项目摘要 转基因小鼠是揭示基本生物学过程和 模拟人类疾病。鼠标模型是该程序不可或缺的元素，使每个 测试假设并模拟人类正常和疾病状态的项目。的主要目标是 实验动物的核心是以最具成本效益和效率的方式为所有项目安置老鼠 交通便利的中央设施。动物核心将继续提供基因分型，帮助计划 与每个研究人员一起进行实验所需的小鼠，并相应地生成适当数量的 小鼠被要求进行拟议的研究。每种品系的小鼠都将被直接饲养和产生 根据调查人员的需要，使用定制软件支持的育种算法。 将使用统计工具来确保使用最少数量的鼠标来提供可报告的 结果。核心将继续进口每个调查员所需的相关品系的小鼠，并将核实 两者的基因分型和保持适当的背景基因株。除了与以下项目相关的活动 小鼠饲养，核心将测试和提供造血细胞转移和 细胞和器官的采集。将为所有转基因动物和 将被安置在核心设施中并在课程期间由所有项目使用的正常对照小鼠品系 这项提议。

Project Summary Genetically engineered mice are critical tools for uncovering basic biological processes and for modeling human disease. Mouse models are an integral element of this program, enabling each of the projects to test hypotheses and model human normal and disease states. The principal goal of the Experimental Animal Core is to house mice for all projects in the most cost effective and efficient manner in a readily accessible central facility. The animal core will continue to provide genotyping, help plan the number of mice required for experiments with each investigator and correspondingly, generate the appropriate number of mice required to carry out the proposed studies. Each strain of mice will be housed and generated in direct proportion to the need of the investigator, with a breeding algorithm that is supported by customized software. Statistical tools will be used to ensure that the fewest number of mice are used that can provide reportable results. The core will continue to import the relevant strains of mice required of each investigator and will verify both genotype and maintain the appropriate background genetic strain. In addition to activities associated with mouse husbandry, the core will test and provide preparative regimens for hematopoietic cell transfers and for cell and organ harvesting. A central registry will be maintained for all genetically engineered animals and normal control mouse strains that will be housed in the core facility and used by all projects during the course of this proposal.