Optical Imaging of Olfactory Sensory Code Transformation
嗅觉感官代码转换的光学成像
基本信息
- 批准号:7902133
- 负责人:
- 金额:$ 31.56万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2008
- 资助国家:美国
- 起止时间:2008-07-01 至 2013-06-30
- 项目状态:已结题
- 来源:
- 关键词:Afferent NeuronsAlzheimer&aposs DiseaseArchitectureBehaviorBrainCalciumCell physiologyCellsCodeDataData SetDendritic CellsDevelopmentDiagnosisDimensionsDiscriminationDiseaseDyesEatingElectrophysiology (science)FlavoringFunctional ImagingFunctional disorderGoalsGrantImageIndividualInstitutesLabelLateralLife StyleLiteratureMapsMethodsMolecular BiologyNeuronsNoseObesityOdorsOlfactory NervePathway interactionsPatternPenetrationPlayPopulationPresynaptic TerminalsProcessPublishingQuality of lifeResearchResearch Project GrantsResolutionRoleSchemeScienceSensorySensory ProcessSignal TransductionSmell PerceptionStagingStructureSurfaceSystemTestingTissuesTransgenic Micebasecharge coupled device cameracombinatorialhuman diseasein vivoinsightinterestmouse modelneuronal cell bodynovelolfactory bulboptical imagingpostsynapticpresynapticpublic health relevancerelating to nervous systemresponsesensorspatiotemporaltwo-photon
项目摘要
DESCRIPTION (provided by applicant): The long-term goal of this research is to understand how the olfactory system encodes odor information, and how olfactory sensory codes are transformed sequentially through different processing stages along the central projection pathways. Olfactory coding and processing have been extensively studied with two major approaches: (1) electrophysiology of single neurons, which can record neural activity at any tissue depth, but blindly without knowing network context in reference to upstream coding patterns; and (2) CCD camera imaging of spatiotemporal pattern of activated glomeruli, which is ideal for revealing the initial glomerulus-based codes, but lacks single-cell resolution and deep penetration required for exploring odor codes beyond the glomerular layer. This grant is aimed at bridging such a gap between single-cell physiology and large-scale CCD camera imaging, so as to unify the two large datasets already available in the literature. First, using a new transgenic mouse model, we will provide a direct comparison between the pre- and postsynaptic odor maps within the glomerular layer, and test the hypothesis that lateral circuits intrinsic to this layer can support interglomerular lateral inhibition and/or excitation for initial odor-map transformation. Second, by combining in vivo two-photon calcium imaging and targeted single-glomerulus dye labeling, we will perform a systematic analysis of odor ensemble codes carried by the mitral/tufted cells associated with a common glomerulus. We will test the hypothesis that both the overall size and distribution pattern of a glomerulus-defined active cell ensemble can be effective coding factors for odor intensity at least and maybe also identity. Finally, by imaging the mitral cell population with diverse glomerular projections, we will analyze the cross-glomerular odor ensemble responses in the context of corresponding glomerular activation patterns. We will study how the glomerulus-based odor codes break down into distributed mitral-cell population codes, and ask what is the benefit of redistributing odor signals which have just converged via the nose-to-bulb projection. Collectively, these studies should not only have a significant impact on our understanding of the neural basis of odor processing and discrimination, but could also yield novel and more general principles on how the brain transforms neural codes for achieving sensory and perceptive functions. PUBLIC HEALTH RELEVANCE The sense of smell plays an important role in our daily life style involving flavor and fragrance appreciation. Dysfunction of the olfactory system happens in many human diseases such as eating-related obesity and early development of Alzheimer's disease. The general goal of this grant in understanding the neural basis of odor coding and processing will not only help the diagnosis and treatment of these diseases, but will also promote people's life quality in general.
描述(由申请人提供):本研究的长期目标是了解嗅觉系统如何编码气味信息,以及嗅觉感觉代码如何沿沿着中枢投射通路通过不同的处理阶段依次转换。嗅觉编码和加工的研究主要有两种方法:(1)单个神经元的电生理学方法,它可以记录任何组织深度的神经活动,但不知道上游编码模式的网络背景;以及(2)激活肾小球的时空模式的CCD相机成像,其对于揭示初始肾小球基代码是理想的,但缺乏探索肾小球层以外的气味代码所需的单细胞分辨率和深度穿透。这项资助旨在弥合单细胞生理学和大规模CCD相机成像之间的差距,以便统一文献中已有的两个大型数据集。首先,使用一种新的转基因小鼠模型,我们将提供一个直接比较的前和突触后的气味地图肾小球层内,并测试假设,这层内在的横向电路可以支持interglomerular横向抑制和/或兴奋的初始气味地图转换。其次,通过结合体内双光子钙成像和有针对性的单肾小球染料标记,我们将进行系统的分析气味合奏代码所进行的二尖瓣/簇状细胞与一个共同的肾小球。我们将测试这一假设,即肾小球定义的活性细胞系综的整体大小和分布模式可以是气味强度的有效编码因素,至少也可能是身份。最后,通过成像的二尖瓣细胞群体与不同的肾小球预测,我们将分析跨肾小球气味合奏的背景下,相应的肾小球激活模式的反应。我们将研究基于肾小球的气味代码如何分解为分布式的线粒体细胞群体代码,并询问重新分配气味信号的好处是什么,这些气味信号刚刚通过鼻子到灯泡的投射会聚。总的来说,这些研究不仅会对我们理解气味处理和辨别的神经基础产生重大影响,而且还可以产生关于大脑如何转换神经代码以实现感官和感知功能的新的和更普遍的原则。公共卫生相关性嗅觉在我们的日常生活方式中起着重要的作用,包括味道和香味的欣赏。嗅觉系统的功能障碍发生在许多人类疾病中,例如与饮食有关的肥胖和阿尔茨海默病的早期发展。这项资助的总体目标是了解气味编码和处理的神经基础,这不仅有助于这些疾病的诊断和治疗,而且还将提高人们的生活质量。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Shin Nagayama其他文献
Shin Nagayama的其他文献
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{{ truncateString('Shin Nagayama', 18)}}的其他基金
Neuronal and Network Dynamics in the Olfactory System
嗅觉系统中的神经元和网络动力学
- 批准号:
8673672 - 财政年份:2014
- 资助金额:
$ 31.56万 - 项目类别:
Neuronal and Network Dynamics in the Olfactory System
嗅觉系统中的神经元和网络动力学
- 批准号:
8827167 - 财政年份:2014
- 资助金额:
$ 31.56万 - 项目类别:
Neuronal and Network Dynamics in the Olfactory System
嗅觉系统中的神经元和网络动力学
- 批准号:
9036994 - 财政年份:2014
- 资助金额:
$ 31.56万 - 项目类别:
Neuronal and Network Dynamics in the Olfactory System
嗅觉系统中的神经元和网络动力学
- 批准号:
9246484 - 财政年份:2014
- 资助金额:
$ 31.56万 - 项目类别:
Differential odor-response and central-projection patterns of mitral and tufted c
二尖瓣和簇绒 c 的差异气味反应和中心投影模式
- 批准号:
7647830 - 财政年份:2009
- 资助金额:
$ 31.56万 - 项目类别:
Differential odor-response and central-projection patterns of mitral and tufted c
二尖瓣和簇绒 c 的差异气味反应和中心投影模式
- 批准号:
7882542 - 财政年份:2009
- 资助金额:
$ 31.56万 - 项目类别:
Differential odor-response and central-projection patterns of mitral and tufted c
二尖瓣和簇绒 c 的差异气味反应和中心投影模式
- 批准号:
8079464 - 财政年份:2009
- 资助金额:
$ 31.56万 - 项目类别:
Optical Imaging of Olfactory Sensory Code Transformation
嗅觉感官代码转换的光学成像
- 批准号:
8089267 - 财政年份:2008
- 资助金额:
$ 31.56万 - 项目类别:
Optical Imaging of Olfactory Sensory Code Transformation
嗅觉感官代码转换的光学成像
- 批准号:
8277392 - 财政年份:2008
- 资助金额:
$ 31.56万 - 项目类别: