Synthetic Modeling of Copper Protein Active Sites

铜蛋白活性位点的综合建模

• 批准号: 7924288
• 负责人: WILLIAM B Tolman
• 金额: $ 3.3万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-30 至 2010-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7924288
• 关键词: Active Sites; Address; Anabolism; Binding; Biological Assay; Biological Process; Biology; Chemicals; Chemistry; Complex; Copper; Data; Decarboxylation; Dioxygen; Disease; Electronics; Electrons; Encapsulated; Enzymes; Glean; Goals; Health; Histidine; Homeostasis; Hormones; Human; Hydroxylation; Ions; Iron; Keto Acids; Knowledge; Laboratories; Lead; Life; Ligands; Metalloproteins; Metals; Methane hydroxylase; Mixed Function Oxygenases; Modeling; Molecular; Molecular Weight; Mono-S; Nature; Neurotransmitters; Nitrites; Nitrogen; Nitrogen Oxides; Nitrous Oxide; Oxidants; Oxidases; Oxidation-Reduction; Oxygen; Particulate; Pathway interactions; Play; Process; Production; Property; Protein Binding; Proteins; Public Health; Reaction; Reagent; Research; Respiration; Respiratory Process; Role; Route; Side; Site; Structure; Structure-Activity Relationship; Sulfides; Sulfur; Testing; Work; analog; biological systems; catalyst; chemical reaction; design; electronic structure; enzyme model; enzyme structure; greenhouse gases; insight; interest; microbial; nitrous oxide reductase; novel; oxidation; oxygen compounds; peptide hormone; protein structure function; public health relevance; small molecule

DESCRIPTION (provided by applicant): Copper plays a key role in numerous environmentally and biologically important processes, particularly when encapsulated within enzymes that are widely distributed in Nature. The copper ions in the active sites of enzymes perform a variety of significant functions, including the binding and activation of dioxygen (O2) for effecting metabolically significant chemical reactions and the reduction of oxidized nitrogen-containing compounds like nitrite and nitrous oxide (N2O) during microbial respiratory processes important within the global nitrogen cycle. Despite extensive research, many questions remain unanswered concerning the detailed molecular level pathways of these processes. The research described herein addresses some of these questions through the synthetic modeling approach. In this approach, low molecular weight complexes designed to replicate aspects of copper enzyme active site structure and function are characterized and their reactivity studied. The goals are to develop detailed understanding of geometries, electronic structures, bonding, and reaction mechanisms relevant to the biological systems. In particular, the research aims to provide detailed understanding of the fundamental chemistry underlying the function of an important subset of copper-containing enzymes involved in the binding and activation of O2 and N2O. This objective will be addressed through three specific aims: (1) Dioxygen Activation at Monocopper Sites, (2) Dioxygen Activation at Multicopper Sites, and (3) Copper-Sulfur Chemistry for Modeling the CuZ Site of Nitrous Oxide Reductase. In aims (1) and (2), synthetic analogs of highly reactive mono- and multicopper oxidizing species will be prepared in order to evaluate their possible role in enzymes that bind and activate O2. In aim (3), new multicopper(I)-sulfide models of the unusual tetracopper-sulfide cluster (CuZ) found in an environmentally important enzyme, nitrous oxide reductase, will be synthesized and their reactivity with N2O will be studied. In addition to aspiring to a deep understanding of copper enzyme structure/function relationships, the proposed work is aimed at developing novel copper chemistry of fundamental significance. PUBLIC HEALTH RELEVANCE: The research aims to provide detailed understanding of the fundamental chemistry underlying the function of an important subset of copper-containing enzymes involved in the binding and activation of O2 and N2O. The dioxygen-activating enzymes are involved in a plethora of important biological processes central to life, including respiration, metal ion homeostasis (the disruption of which causes disease), and the production of important organic metabolites, hormones, and neurotransmitters essential to human health. The reduction of N2O by the microbial enzyme nitrous oxide reductase converts this greenhouse gas to inert N2 in a process recognized to be a critical component of the global nitrogen cycle and thus directly relevant to public health.

描述（由申请人提供）：铜在许多环境和生物重要过程中起着关键作用，特别是当被封装在自然界广泛分布的酶中时。在酶的活性位点中的铜离子执行各种重要功能，包括结合和活化双氧（O2）以实现代谢上重要的化学反应，以及在全球氮循环中重要的微生物呼吸过程期间还原氧化的含氮化合物如亚硝酸盐和一氧化二氮（N2 O）。尽管进行了广泛的研究，但关于这些过程的详细分子水平途径的许多问题仍然没有答案。本文所述的研究通过综合建模方法解决了其中的一些问题。在这种方法中，低分子量的配合物，旨在复制铜酶活性位点的结构和功能方面的特点，并研究其反应性。目标是发展对与生物系统相关的几何结构，电子结构，键合和反应机制的详细理解。特别是，该研究旨在详细了解参与O2和N2 O结合和激活的含铜酶的重要子集的基本化学功能。这一目标将通过三个具体目标来实现：（1）单铜位点的分子氧活化，（2）多铜位点的分子氧活化，以及（3）用于模拟一氧化二氮还原酶CuZ位点的铜硫化学。在目标（1）和（2）中，将制备高反应性单铜和多铜氧化物质的合成类似物，以评估它们在结合和活化O2的酶中的可能作用。在目标（3）中，将合成在环境重要酶一氧化二氮还原酶中发现的不寻常的四铜硫化物簇（CuZ）的新的多铜（I）硫化物模型，并将研究它们与N2 O的反应性。除了渴望深入了解铜酶的结构/功能关系外，拟议的工作旨在开发具有根本意义的新型铜化学。公共卫生关系：该研究旨在详细了解参与O2和N2 O结合和激活的含铜酶的重要子集的基本化学功能。双氧激活酶参与了许多重要的生物过程，包括呼吸、金属离子稳态（破坏金属离子稳态会导致疾病）以及重要的有机代谢物、激素和对人类健康至关重要的神经递质的产生。通过微生物酶一氧化二氮还原酶还原N2 O，将这种温室气体转化为惰性N2，这一过程被认为是全球氮循环的关键组成部分，因此直接关系到公众健康。