REPLICATION OF NOROVIRUSES IN CELL CULTURE

诺病毒在细胞培养中的复制

• 批准号: 7959698
• 负责人: Kyeong-Ok Chang
• 金额: $ 25.35万
• 依托单位: UNIVERSITY OF KANSAS MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7959698
• 关键词: Acute; Antiviral Agents; Bile Acids; Calicivirus; Categories; Cell Culture System; Cell Culture Techniques; Cells; Cholesterol; Computer Retrieval of Information on Scientific Projects Database; Development; Disease; Disease Outbreaks; Enteral; Family suidae; Funding; G-Protein-Coupled Receptors; Gastroenteritis; Goals; Grant; Institution; Measures; National Institute of Allergy and Infectious Disease; Norovirus; Norwalk virus; Pathway interactions; Prevention strategy; Preventive; Public Health; Replicon; Research; Research Personnel; Resources; Source; System; United States National Institutes of Health; Virus; base; microbial; novel; novel strategies; pathogen; receptor; vaccine development

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Although noroviruses (NIAID category B priority pathogen) are a major public health concern with their ability to cause large outbreaks of acute gastroenteritis, the lack of a cell culture system greatly hinders research on preventive measures, such as the developments of vaccines and antiviral drugs. The long term goal of this application is to understand the replication of noroviruses. Novel cell based norovirus replication system (Norwalk virus [NV] replicon-bearing cells), which we developed recently, will be used for studying replication and antivirals of noroviruses. Especially we will focus on the effects of IFNs on the replication of NV. We plan to generate additional norovirus replicon-bearing cells using different norovirus strains adapting the similar strategy for that of NV replicon bearing cells in searching for strains efficiently replicate in cells. Because we found the bile acids and cholesterol pathways were important in growing the related virus, porcine enteric calicivirus (PEC) in cell culture, we will examine bile acids/cholesterol pathways in the replication of norovirus. Specifically, we will examine farnesoid X receptor (FXR) and G protein couple receptor (TGR5) of bile acids in the replication of norovirus. These studies should contribute to the isolation of noroviruses in cell culture, which is a key for development of preventive strategies to control diarrheal disease caused by noroviruses.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 尽管诺如病毒（NIAID B类病原体）是引起大量急性胃肠炎暴发的能力的主要公共卫生问题，但缺乏细胞培养系统极大地阻碍了人们对预防措施的研究，例如疫苗的发展和抗病毒药物。该应用程序的长期目标是了解诺诺病毒的复制。我们最近开发的新型基于细胞的诺如病毒复制系统（Norwalk病毒[NV]复制细胞）将用于研究北病毒的复制和抗病毒药物。特别是我们将重点关注IFN对NV复制的影响。我们计划使用不同的诺如病毒菌株生成其他诺如病毒复制子的细胞，从而适应NV复制子轴承细胞的相似策略，以在细胞中有效地复制菌株。因为我们发现胆汁酸和胆固醇途径对于在细胞培养中种植相关病毒，猪肠胃病毒（PEC）很重要，所以我们将检查诺如病毒复制中的胆汁酸/胆固醇途径。具体而言，我们将在诺如病毒复制中检查胆汁酸的Farnesoid X受体（FXR）和G蛋白夫妇受体（TGR5）。这些研究应促进细胞培养中诺诺未病毒的隔离，这是制定控制诺病毒引起的腹泻疾病的预防策略的关键。