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The Mechanistic Basis of Cyclin A2-Mediated Cardiac Repair

细胞周期蛋白 A2 介导的心脏修复的机制基础

基本信息

批准号：
7903998
负责人：
Hina W Chaudhry
金额：
$ 42.38万
依托单位：
ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-04-15 至 2012-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7903998
关键词：
Adenoviruses Adult Arts Biomedical Engineering Bone Marrow Bromodeoxyuridine Cardiac Cardiac Myocytes Cardiac Output Cardiology Cardiovascular Diseases Cell Cycle Cell Cycle Arrest Cell Proliferation Cell Transplantation Cells Cellular biology Characteristics Cicatrix Clinical Coculture Techniques Complementary DNA Cyclin-Dependent Kinases Cyclins Cytokinesis Developmental Biology Embryo Engineering Exhibits Gene Transfer Genetic Heart Heart failure In Vitro Infarction Injection of therapeutic agent Injury Investigation Life Magnetic Resonance Imaging Mammalian Cell Measures Mediating Mitosis Mitotic Modeling Monitor Mus Muscle Cells Myocardial Infarction Myocardium Myosin Heavy Chains Natural regeneration Neonatal Newts Operative Surgical Procedures Organogenesis Play Population Proliferating Radiology Specialty Rattus Recovery Reporter Genes Research Personnel Research Training Resources Role Side Silicon Dioxide Site Source Stem cells Techniques Testing Therapeutic Time Tissue Engineering Tissues Transgenic Mice Transgenic Organisms Translations Transplantation Ventricular Cardiac alpha-Myosin Video Microscopy Viral base cell dedifferentiation cell type cyclin A2 in vivo insight interdisciplinary approach mouse model novel postnatal progenitor programs promoter repaired response response to injury stem cell population tool uptake

项目摘要

The concept of stimulating cardiomyocyte mitotic divisions has dramatic implications for cardiac regeneration and hence, cardiovascular disease. We previously demonstrated for the first time that mammalian cardiomyocyte mitoses can occur postnatally if cyclin A2 is constitutively expressed in cardiomyocytes in a transgenic mouse model. Our more recent results demonstrate that cyclin A2 mediates cardiomyocyte mitoses in the transgenic hearts after myocardial infarction (Ml) is induced, thus repairing the heart with significant recovery of cardiac function when compared with nontransgenic littermate controls. To explore potential clinical translation of these results, we administered cDNA encoding cyclin A2 via de-activated adenovirus to infarcted rats and noted that the rats receiving adeno-cyclin A2 undergo cellular regeneration at the sites of injection and have preserved cardiac output when compared to controls which received empty adenovirus. We hypothesize that there are two potential mechanisms mediating the regeneration we have observed. First, side population (SP) stem cells appear to differentiate into cardiomyocytes in our mouse infarction model, but mitoses in these immature cardiomyocytes are only noted in the transgenic mice, thus possibly uncovering a novel mechanism to induce hyperproliferative progenitor cells. Second, we note a significant increase in expression of phosphohistone H3 and uptake of BrdU in the peri-infarct zone of transgenic mice compared to nontransgenic controls, implicating dedifferentiation and division of adult cardiomyocytes. Utilizing SP cells from transgenic and nontransgenic mice, we propose to explore the first mechanism via in vitro studies of proliferation of SP cell-derived progenitor cells and in vivo studies of SP cell transplantation into a mouse infarction model. Additionally, we will use novel engineered cardiac tissues to evaluate both mechanisms by testing the response to injury of both SP cell-derived progenitors and adult cardiomyocytes from transgenic and nontransgenic mice. The second mechanism will also be examined using viral transfer of cyclin A2 cDNA into adult cardiomyocytes in vitro and the utilization of video microscopy to monitor mitosis and cytokinesis of these cells. These studies are proposed in order to elucidate a more directed path to clinical therapy.

刺激心肌细胞有丝分裂的概念对心脏再生以及心血管疾病具有显著的意义。我们先前第一次证明，哺乳动物心肌细胞有丝分裂可以发生产后，如果细胞周期蛋白A2是组成型表达的转基因小鼠模型中的心肌细胞。我们最近的研究结果表明，细胞周期蛋白A2介导的心肌细胞有丝分裂在转基因心脏心肌梗死（MI）诱导后，从而修复心脏与显着恢复的心脏功能相比，非转基因同窝对照。为了探索这些结果的潜在临床转化，我们通过灭活腺病毒向梗死大鼠给予编码细胞周期蛋白A2的cDNA，并注意到接受腺细胞周期蛋白A2的大鼠在注射部位进行细胞再生，并且与接受空腺病毒的对照组相比，保留了心输出量。我们推测，有两个潜在的机制介导的再生，我们已经观察到。首先，侧群（SP）干细胞在我们的小鼠梗死模型中分化为心肌细胞，但这些未成熟心肌细胞中的有丝分裂仅在转基因小鼠中观察到，因此可能揭示了诱导过度增殖祖细胞的新机制。第二，我们注意到磷酸化组蛋白H3的表达和BrdU的摄取在转基因小鼠的梗死周围区相比，nontransgenic控制，涉及成年心肌细胞的去分化和分裂的显着增加。利用转基因和非转基因小鼠的SP细胞，我们建议通过SP细胞衍生的祖细胞增殖的体外研究和SP细胞移植到小鼠梗死模型的体内研究来探索第一种机制。此外，我们将使用新的工程心脏组织，以评估这两种机制，通过测试SP细胞衍生的祖细胞和成年心肌细胞从转基因和非转基因小鼠的损伤的反应。第二种机制也将使用病毒转移到成人心肌细胞的细胞周期蛋白A2的cDNA在体外和利用视频显微镜监测这些细胞的有丝分裂和胞质分裂进行检查。提出这些研究是为了阐明一个更直接的临床治疗途径。