Physiology and Pathophysiology of the Primate Gonad

灵长类性腺的生理学和病理生理学

• 批准号: 7932569
• 负责人: TONY M PLANT
• 金额: $ 23.57万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-30 至 2011-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7932569
• 关键词: Physiology; Pathophysiology; Primate; Gonad

DESCRIPTION (provided by applicant): The mission of the Specialized Cooperative Centers Program in Reproduction Research (SCCPRR) at the University of Pittsburgh School of Medicine continues to be the study of the physiology (integrative genomics) of the control systems that govern gonadal function in higher primates, including man. This mission of the Pittsburgh SCCPRR, which reflects the long-standing forte of this Center to employ non-human primate models to better understand human reproduction, is pursued by integrating molecular, cellular and system approaches. The Center will be comprised of two Technical Service Cores (Primate and Assay) with an open access format to sub serve six SCCPRR projects (four in Pittsburgh), three projects at the Cooperative Reproductive Sciences Center at Morehouse School of Medicine, and several other programs supported by either R01 or R21 grants. Three of the four Pittsburgh SCCPRR projects focus on the development and control of the testes, with a particular emphasis on the regulation of proliferation and differentiation of stem spermatogonia (male germ-line stem cells, GSCs) and their niche providing somatic cells of Sertoli. Studies of premeiotic spermatogonia will be restricted to the human and monkey testes, and although the molecular biology underlying Sertoli cell proliferation and differentiation will be elucidated with non-primate models, we aim to concomitantly translate findings in the rodent to the monkey. The fourth project will examine the control mechanisms that govern the early stages of follicular development using a non-human primate model. Both conceptual and methodological bridges link the four projects. In this regard, there are parallels in the paracrine control of follicular development, on the one hand, and the regulation of the male germinal epithelium, on the other. At the technical level, methodology developed at the Pittsburgh Center for controlling gene expression in granulosa cells will find direct application to the study of Sertoli cell biology in this proposal. The theme of the primate gonad is explored with vertical balance in investigator experience, and it is anticipated that this approach will guarantee that the Pittsburgh SCCPRR will continue to contribute significantly to our understanding of human reproduction and to the treatment of its disorders.

描述（由申请人提供）：匹兹堡大学医学院的专业合作中心计划（SCCPRR）的任务继续是对控制高级灵长类动物（包括男人）在包括男人（包括男子）中贡达数字的控制系统的生理学（综合基因组学）的研究。匹兹堡SCCPRR的这一使命反映了该中心长期使用非人类灵长类动物模型来更好地理解人类繁殖的过程，它是通过整合分子，细胞和系统方法来追求的。该中心将由两个技术服务核心（灵长类动物和测定）组成，并采用开放访问格式，可为六个SCCPRR项目（匹兹堡的四个），莫尔豪斯医学院合作生殖科学中心的三个项目，以及其他几个由R01或R21赠款支持的计划。匹兹堡SCCPRR的四个项目中有三个侧重于睾丸的发育和控制，特别着重于调节茎精子的增殖和分化（男性种系干细胞，GSC，GSCS）及其利基市场，提供了Sertoli的体细胞。对近去的精子症的研究将仅限于人类和猴子睾丸，尽管Sertoli细胞增殖和分化的分子生物学将与非青调模型阐明，但我们的目的是将啮齿动物中的发现同时转化为猴子。第四个项目将检查使用非人类灵长类动物模型来控制卵泡发展早期阶段的控制机制。概念和方法论桥都将四个项目联系起来。在这方面，另一方面，在卵泡发育的旁分泌控制中有相似之处，另一方面是对雄性生发性上皮的调节。在技​​术层面，在匹兹堡控制颗粒细胞中基因表达的方法中开发的方法学将直接应用于该提案中的Sertoli细胞生物学研究。在调查员的经验中，探索了灵长类动物的主题，并预计这种方法将保证匹兹堡SCCPRR将继续为我们对人类繁殖以及对其疾病的治疗做出重大贡献。