Triglyceride Rich Lipoprotein Metabolism and Fatty Acid Selectivity

富含甘油三酯的脂蛋白代谢和脂肪酸选择性

• 批准号: 7806205
• 负责人: ROBERT Henry NELSON
• 金额: $ 0.11万
• 依托单位: MAYO CLINIC ROCHESTER
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-30 至 2013-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7806205
• 关键词: Abdomen; Address; Adipocytes; Adipose tissue; Appearance; Behavior; Blood Pressure; Chylomicrons; Communities; Development; Diabetes Mellitus; Dilution Techniques; Emulsions; Enteral; Epinephrine; Etiology; Fatty Acids; Functional disorder; Glucose; Goals; High Pressure Liquid Chromatography; Hormones; Human; Infusion procedures; Ingestion; Insulin; Intravenous infusion procedures; Investigation; Knowledge; Label; Lipids; Lipolysis; Lipoproteins; Measurement; Measures; Metabolic; Metabolism; Muscle; Non-Insulin-Dependent Diabetes Mellitus; Nonesterified Fatty Acids; Oral; Palmitates; Pancreas; Pharmacologic Substance; Plasma; Plethysmography; Polyunsaturated Fatty Acids; Prevention; Process; Research Personnel; Saline; Saturated Fatty Acids; Structure; Testing; Tracer; Triglycerides; Tumor Necrosis Factor-alpha; Unsaturated Fatty Acids; adiponectin; base; extracellular; fatty acid metabolism; inflammatory marker; insulin sensitivity; lipid metabolism; lipoprotein lipase; liquid chromatography mass spectroscopy; obesity treatment; research study; skills; subcutaneous; uptake

DESCRIPTION (provided by applicant): Understanding the details of metabolism of lipids in normal humans is essential to the development of effective dietary and pharmaceutical prevention and treatment of obesity and type 2 diabetes. The fundamental processes of fatty acid release from adipocytes, the uptake of plasma free fatty acids by muscle and adipose tissue and the contribution of extracellular lipolysis of triglyceride rich lipoproteins to the free fatty acid pool are only partially understood. Epidemiologic and dietary studies suggest that there are differences in fatty acid metabolism based on fatty acid structure (saturated, monounsaturated or polyunsaturated). This proposal addresses gaps in the knowledge of fatty acid metabolism by testing the following hypotheses: 1. Oleate and palmitate generated by lipoprotein lipase lipolysis of circulating triglycerides spill over into the plasma free fatty acid pool more than linoleate. 2. Adipose tissue releases fatty acids selectively. 3. Changes in insulin sensitivity, endothelial function and blood pressure vary by the structure of fatty acids infused enterally to elevate free fatty acids. These hypotheses will be tested using triglyceride and fatty acid tracer dilution techniques, high performance liquid chromatography and mass spectroscopy. Two of the studies will be done using intravenous infusion tracers with one study also using oral lipid tracers. Specific aim 1 uses tracers with a mixed meal, specific aim 2 utilizes tracers with hormone infusions and specific aim 3 uses oral unlabeled fatty acid infusion in the form of a triglyceride with a pure fatty acid structure with measures of endothelial dysfunction, blood pressure, insulin sensitivity and changes in inflammatory markers compared to saline ingestion. These studies will provide the applicant with an opportunity to develop further the skills necessary to achieve his long-term goal of becoming an independent investigator exploring the relationship between lipid metabolism and diabetes.

描述（由申请人提供）：