VECTOR-BORNE DISEASES CORE

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 VBD核心区继续维持斯氏按蚊种群，该种群被用于将食蟹猴疟原虫（人类间日疟的一种模型）传播给恒河猴。蜱虫菌落继续发挥作用，使我们的部门能够利用自然感染模式研究莱姆病。我们现有的蜱若虫毛细血管饲养技术，使我们能够用螺旋体克隆分离株感染蜱。这通常是必要的，以确保明确的宿主对感染的反应。我们也可以通过浸泡在含有螺旋体的组织培养液中来感染蜱虫幼虫。核心的蜱虫部分目前有许多肩胛硬蜱所有发育阶段的标本。幼虫、幼虫和成虫以交错的方式储存在4 ℃下。因此，我们通常在全年的大多数时间都有所有的阶段。这包括5-10罐幼虫（每罐约1000只幼虫）和数百只未感染的幼虫，以备实验需要。今年，我们继续收集蜱唾液，以研究其对B诱导的炎症反应的影响。当伯氏螺旋体侵入宿主皮肤时，我们还继续与德克萨斯州休斯顿的德克萨斯大学健康科学中心合作，鉴定B的毒力决定因素。影响螺旋体对蜱的感染性，以及通过蜱对小鼠的感染性。今年收集了46只成年雌性并进行了血液喂养。总共有27只成功喂食，大多数都产卵了。喂食和收集的幼虫数量为866只，其中660只成功蜕皮为幼虫，用于未来的实验。 共194只大鼠成功地进行了毛细管饲养。这些蜱用于3个大型独立实验。剩余的现金流量将于本年度使用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The VBD core has continued to maintain an Anopheles stephensi colony, which is used to transmit Plasmodium cynomolgi, a model of human vivax malaria, to rhesus macaques. The tick colony has continued to be instrumental in enabling our division to do research in Lyme borreliosis using the natural mode of infection. The technique of capillary feeding of nymphal ticks, which we have available, allows us to infect ticks with spirochetal clonal isolates. This is often essential to insure defined host-responses to infection. We also are able to infect larval ticks by immersion in tissue-culture fluid that contains spirochetes. The tick section of the core has currently available numerous specimens of all of the developmental stages of Ixodes scapularis. Larvae, nymphs, and adults are stored at 4¿C in a staggered fashion. Therefore, we usually have all of the stages available at most times throughout the year. This includes 5-10 jars of larvae (with about 1000 larvae each) and several hundred uninfected nymphs for experimental needs as they arise. This year we have continued with the collection of tick saliva, to study its effects on the inflammatory response induced by B. burgdorferi spirochetes as they invade the host skin. We also have continued our collaboration with the University of Texas Health Sciences Center, Houston, TX, on the identification of virulence determinants of B. burgdorferi the affect infectivity of spirochetes to ticks, and to mice via ticks. This year 46 adult females were collected and were blood fed. A total of 27 of those fed successfully, and most laid eggs. The number of larvae that fed and were collected were 866, and 660 of those successfully molted into nymphs to be used in future experiments. A total of 194 nymphs were capillary-fed successfully. Those ticks were used in 3 large, separate experiments. The remainder of the nymphs will be used in the current year.