VECTOR-BORNE DISEASES CORE

媒介传播疾病核心

• 批准号: 8358066
• 负责人: MARIO TOMAS PHILIPP
• 金额: $ 3.72万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8358066
• 关键词: Adult; Affect; Anopheles Genus; Black-legged Tick; Blood; Blood capillaries; Borrelia burgdorferi; Collaborations; Development; Ensure; Female; Funding; Future; Grant; Health Sciences; Human; Immersion Investigative Technique; Immune response; Infection; Larva; Liquid substance; Lyme Disease; Macaca mulatta; Modeling; Molting; Mus; National Center for Research Resources; Nymph; Order Spirochaetales; Plasmodium cynomolgi; Primates; Principal Investigator; Research; Research Infrastructure; Resources; Source; Specimen; Staging; Techniques; Texas; Ticks; Time; United States National Institutes of Health; Universities; Virulence; Vivax Malaria; capillary; cost; egg; feeding; research study; tissue culture

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The VBD core has continued to maintain an Anopheles stephensi colony, which is used to transmit Plasmodium cynomolgi, a model of human vivax malaria, to rhesus macaques. The tick colony has continued to be instrumental in enabling our division to do research in Lyme borreliosis using the natural mode of infection. The technique of capillary feeding of nymphal ticks, which we have available, allows us to infect ticks with spirochetal clonal isolates. This is often essential to ensure defined host-responses to infection. We also are able to infect larval ticks by immersion in tissue-culture fluid that contains spirochetes. The tick section of the core has currently available numerous specimens of all of the developmental stages of Ixodes scapularis. Larvae, nymphs, and adults are stored at 4¿C in a staggered fashion. Therefore, we usually have all of the stages available at most times throughout the year. This includes 5-10 jars of larvae (with about 1000 larvae each) and several hundred uninfected nymphs for experimental needs as they arise. We have continued our collaboration with the University of Texas Health Sciences Center, Houston, TX, on the identification of virulence determinants of B. burgdorferi the affect infectivity of spirochetes to ticks, and to mice via ticks. This year 81 adult females were collected and were blood fed. A total of 51 of those fed successfully, and 36 of those laid eggs. The number of larvae that fed and were collected were 1982, and 1535 of those successfully molted into nymphs to be used in future experiments. A total of 497 nymphs were capillary-fed successfully. Those ticks were used in 3 large experiments. The remainder of the nymphs will be used in the current year.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 VBD 核心继续维持史氏按蚊群落，用于将食蟹猴疟原虫（一种人类间日疟疾模型）传播给恒河猴。蜱群继续在使我们部门能够利用自然感染方式研究莱姆疏螺旋体病方面发挥着重要作用。我们现有的若虫蜱的毛细管喂养技术使我们能够用螺旋体克隆分离株感染蜱。这对于确保明确的宿主对感染的反应通常至关重要。我们还可以通过浸入含有螺旋体的组织培养液中来感染蜱幼虫。核心的蜱部分目前有肩胛硬蜱所有发育阶段的大量标本。幼虫、若虫和成虫以交错方式储存在 4°C 下。因此，我们通常在一年中的大部分时间都提供所有舞台。其中包括 5-10 罐幼虫（每罐约 1000 只幼虫）和数百只未感染的若虫，以满足实验需要。我们继续与德克萨斯州休斯顿的德克萨斯大学健康科学中心合作，鉴定伯氏疏螺旋体的毒力决定因素，影响螺旋体对蜱虫的感染性，以及通过蜱虫对小鼠的感染性。今年收集了 81 只成年雌性并进行了血液喂养。其中 51 只成功进食，其中 36 只产卵。进食并收集的幼虫数量为 1982 只，其中 1535 只成功蜕变成若虫，用于未来的实验。 共有497只若虫被成功毛细管喂养。这些蜱虫被用于 3 个大型实验。剩余的若虫将在当年使用。