PROTEIN & NUCLEIC ACID CORE FACILITY

蛋白质

• 批准号: 7959231
• 负责人: KRISHNA BAKSI
• 金额: $ 12.77万
• 依托单位: UNIVERSIDAD CENTRAL DEL CARIBE
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-15 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7959231
• 关键词: AIDS/HIV problem; Address; Area; Behavioral; Biological; Biomedical Research; Blood Platelets; Cancer Biology; Caveolae; Caveolins; Cell physiology; Cells; Cellular biology; Chimeric Proteins; Collaborations; Core Facility; DNA Vaccines; Data; Educational workshop; Endothelial Cells; Equipment; Faculty; Funding; Future; Genes; Grant; Hand; Immune system; Institutes; Institution; Journals; Maintenance; Microgravity; Molecular; Molecular Biology Techniques; Muscle; Nanotechnology; Neurons; Neuropharmacology; Nucleic Acid Core Facility; Nucleic Acids; Parkinson Disease; Peer Review; Postdoctoral Fellow; Protein Analysis; Proteins; Publications; Publishing; Puerto Rico; Research; Research Personnel; Resources; Services; Source; Steroids; Techniques; Toxin; Training; Universities; Vaccine Production; base; computerized; cost; medical schools; member; protein expression; protein purification; receptor; research and development

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The research performed at the UCC medical school are in different fields from behavioral neuropharmacology, repair mechanism, kinetics of DNA damage, neuronal and muscular receptor studies, cellular physiology and the analysis of toxin with its receptor. The Protein and Nucleic Acid Core Facility (PNACF) of the CMBC likes to aid in the transition from the more classical studies to a more molecular and detailed analysis of the problems being addressed by its faculty members. PNACF will focus on faculty training, seminars, training on specialized molecular biology techniques, protein expression and protein purification and characterization. Therefore, the PNACF continues to be an important core facility for the maintenance and future development of research at this institution. The SPECIFIC AIMS for improving this facility are: 1. to continue to acquire the molecular tools needed to analyze basic biomedical problems in a more detailed and modern fashion. (2). to sponsor "hand-on' workshops in molecular biological techniques and protein expression and protein purification techniques given by invited experts of the fields. This will help the UCC researchers to solve their problem and to obtain preliminary data in their areas of their research. (3) to have the facility for the protein expression and purification in the UCC, and to encourage the UCC faculty members to expand their research to the protein level. (4) to continue and expand the resource centre where UCC faculty can obtain technical informations and cost estimations for molecular biological techniques and services which are not available in the UCC. (5) to continue and improve the computerized analysis of nucleic acid and protein data, and (6) to implement a "fee for service" plan that will be used for the service contract/repair for VersaDOC system of Bio-RAD. This is to start to get partial self-support for the PNACF component of the RCMI program.

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 UCC医学院的研究从行为神经药理学、修复机制、DNA损伤动力学、神经元和肌肉受体研究、细胞生理学以及毒素及其受体的分析等不同领域进行。CMBC的蛋白质和核酸核心设施(PNACF)喜欢帮助从更经典的研究过渡到对其教职员工正在解决的问题进行更分子和详细的分析。PNACF将专注于教师培训、研讨会、专门的分子生物学技术、蛋白质表达和蛋白质纯化和表征方面的培训。因此，PNACF仍然是该机构维护和未来研究发展的重要核心设施。改进这一设施的具体目标是：1.继续获得以更详细和现代的方式分析基本生物医学问题所需的分子工具。(2)。赞助由该领域特邀专家举办的分子生物技术、蛋白质表达和蛋白质纯化技术方面的“动手”工作坊。这将帮助UCC的研究人员解决他们的问题，并在他们的研究领域获得初步数据。(3)拥有在UCC表达和纯化蛋白质的设施，并鼓励UCC教职员工将他们的研究扩展到蛋白质水平。(4)继续和扩大UCC教职员工可以获得UCC没有的分子生物技术和服务的技术信息和成本估算的资源中心。(5)继续并改进核酸和蛋白质数据的计算机化分析；(6)实施将用于Bio-RAD VersaDOC系统服务合同/维修的“服务费”计划。这是为了开始对RCMI计划的PNACF组件进行部分自我支持。