PROTEIN & NUCLEIC ACID CORE FACILITY

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 在UCC医学院进行的研究涵盖了广泛的领域，包括行为神经药理学，神经元和肌肉受体研究细胞生理学，神经元和神经胶质网络，内皮细胞生物学，免疫系统中的类固醇作用和微重力对免疫系统的影响，小窝和小窝蛋白表达和毒素及其受体的分析，帕金森病，DNA疫苗生产，癌症生物学和血小板功能。 蛋白质和核酸核心设施（PNACF）将帮助UCC研究人员从更经典的研究过渡到对每个研究人员正在解决的问题进行更分子和最新的分析。 PNACF将专注于教师培训，研讨会，专业分子生物学技术，蛋白质表达和蛋白质纯化和表征的培训。 因此，PNACF仍然是该机构研究的维护和未来发展的重要核心设施。具体目标是： (1)继续帮助研究人员培训/服务当前的分子生物学技术，并培训其他研究人员，包括博士后研究员和研究助理，使用和维护专业设备。 (2)与美国本土其他机构的核心设施合作，引进两项强大的技术，即基因微阵列和纳米技术。 (3)赞助分子生物学技术、无细胞蛋白质表达和融合蛋白纯化技术的“动手”讲习班，由这些领域的受邀专家讲授。 这将有助于UCC研究人员直接解决他们的研究问题，并获得出版物/演示文稿/外部资金的初步数据。 (4)继续成为一个资源中心，UCC教师可以获得技术信息和成本估算的分子生物学/蛋白质分析技术和服务，在UCC不可用。 (5)继续帮助研究人员进行核酸和蛋白质数据的计算机分析。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The research performed at the UCC Medical School covers a wide range of areas including behavioral neuropharmacology, neuronal and muscular receptor studies to cellular physiology, neuronal and glial networking, endothelial cell biology, steroid actions in the immune system and the effect of microgravity on the immune system, caveolae and caveolin expression and the analysis of toxin with its receptor, Parkinson's Disease, DNA vaccine production, cancer biology and platelet function. The Protein and Nucleic Acid Core Facility (PNACF) will aid the UCC investigators in the transition from the more classical studies to a more molecular and up-to-date analysis of the problems being addressed by each investigators. PNACF will focus on faculty training, seminars, training on specialized molecular biology techniques, protein expression and protein purification and characterization. Therefore, the PNACF continues to be an important core facility for the maintenance and future development of research at this institution. Specific Aims are: (1) To continue helping researchers with training/service current molecular biology techniques and training other researchers including post-doctoral fellows and research assistants, on the use and maintenance of specialized equipments. (2) To introduce two powerful techniques, namely gene microarray and nanotechnology in collaboration with the core facilities of other institutions in the mainland USA. (3) To sponsor 'hand-on" workshops in molecular biological techniques, cell-free protein expression and fusion protein purification techniques given by the invited experts in each of these fields. This will help the UCC researchers to directly address their research problems and to obtain preliminary data for publications/presentations/external funding. (4) To continue to be a resource center where the UCC faculty can obtain technical information and cost estimation for molecular biological/protein analysis techniques and services that are not available at UCC. (5) To continue helping the researchers for the computerized analysis of nucleic acid and protein data.