PROTEIN & NUCLEIC ACID CORE FACILITY

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 UCC医学院进行的这项研究涵盖了广泛的领域，包括行为神经药理学、神经和肌肉受体研究、细胞生理学、神经元和神经胶质网络、内皮细胞生物学、免疫系统中的类固醇作用和微重力对免疫系统的影响、小窝和小窝的表达以及毒素及其受体的分析、帕金森氏病、DNA疫苗生产、癌症生物学和血小板功能。蛋白质和核酸核心设施(PNACF)将帮助UCC调查人员从更经典的研究过渡到对每个调查人员正在解决的问题进行更分子和最新的分析。PNACF将专注于教师培训、研讨会、专门的分子生物学技术、蛋白质表达和蛋白质纯化和表征方面的培训。因此，PNACF仍然是该机构维护和未来研究发展的重要核心设施。具体目标是： (1)继续帮助研究人员培训/服务当前的分子生物学技术，并培训其他研究人员，包括博士后研究员和研究助理，了解专业设备的使用和维护。 (2)与美国内地其他机构的核心设施合作，引进两项强大的技术，即基因微阵列和纳米技术。 (3)赞助分子生物学技术、无细胞蛋白表达和融合蛋白纯化技术方面的“实践”工作坊，由这些领域的特邀专家举办。这将帮助UCC的研究人员直接解决他们的研究问题，并为出版/演讲/外部资助获得初步数据。 (4)继续成为UCC教职员工可以获得UCC没有的分子生物/蛋白质分析技术和服务的技术信息和成本估算的资源中心。 (5)继续协助研究人员对核酸和蛋白质数据进行计算机分析。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The research performed at the UCC Medical School covers a wide range of areas including behavioral neuropharmacology, neuronal and muscular receptor studies to cellular physiology, neuronal and glial networking, endothelial cell biology, steroid actions in the immune system and the effect of microgravity on the immune system, caveolae and caveolin expression and the analysis of toxin with its receptor, Parkinson's Disease, DNA vaccine production, cancer biology and platelet function. The Protein and Nucleic Acid Core Facility (PNACF) will aid the UCC investigators in the transition from the more classical studies to a more molecular and up-to-date analysis of the problems being addressed by each investigators. PNACF will focus on faculty training, seminars, training on specialized molecular biology techniques, protein expression and protein purification and characterization. Therefore, the PNACF continues to be an important core facility for the maintenance and future development of research at this institution. Specific Aims are: (1) To continue helping researchers with training/service current molecular biology techniques and training other researchers including post-doctoral fellows and research assistants, on the use and maintenance of specialized equipments. (2) To introduce two powerful techniques, namely gene microarray and nanotechnology in collaboration with the core facilities of other institutions in the mainland USA. (3) To sponsor 'hand-on" workshops in molecular biological techniques, cell-free protein expression and fusion protein purification techniques given by the invited experts in each of these fields. This will help the UCC researchers to directly address their research problems and to obtain preliminary data for publications/presentations/external funding. (4) To continue to be a resource center where the UCC faculty can obtain technical information and cost estimation for molecular biological/protein analysis techniques and services that are not available at UCC. (5) To continue helping the researchers for the computerized analysis of nucleic acid and protein data.