INSULINOPENIC TYPE 2 DIABETES IN HISPANICS

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 我们的具体目标是检验以下假设：在洛杉矶南湾地区有一个独特的人群，他们患有胰岛素减少性DM-2，可通过他们的2型糖尿病酮症酮症酸中毒综合征（SKIT-2）来识别。我们的第一个目的是检验这一假设，即这些患者代表了较大的2型糖尿病患者人群中的一个独特的流行病学亚组。我们假设，与未发生酮症酸中毒的匹配DM-2受试者相比，SKIT-2患者在DKA后3个月随访时将继续显示胰岛素减少。这将通过对胰岛素分泌和敏感性的心理学研究进行测试。在这些研究中，我们将重点关注西班牙裔受试者，以便在研究的受试者中获得一定程度的同质性。一些受试者将在已知的糖尿病基因中发生突变。这将通过筛选SKIT-2先证者的线粒体DNA和HNF-1 α突变进行评估。一旦该项目获得资助，也将对先证者进行HLA分型。在此之前，将储存DNA和/或获得淋巴细胞并转化用于后续研究。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Our specific aims are to test the following hypotheses: There is a distinct population in the Los Angeles South Bay area that have insulinopenic DM-2, identifiable by their presentation with ketoacidosis Syndrome of Ketosis In Type 2 Diabetes, SKIT-2. Our first aim is to test the hypothesis that these patients represent a distinct epidemiological subgroup within the larger patient population of type 2 diabetes mellitus. We hypothesize that patients with SKIT-2 will continue to show insulinopenia at follow up over 3 months after DKA, when compared with matched DM-2 subjects who have not experienced ketoacidosis. This will be tested using psychological studies of insulin secretion and sensitivity. In these studies we will focus upon Hispanic subjects so as to obtain a degree of homogeneity in the subjects studied. Some subjects will have a mutation in a known diabetes gene. This will be assessed by screening SKIT-2 probands for mutations in mitochondrial DNA and HNF-1alpha. HLA typing of probands will also be done once this project is funded. Until then, DNA will be stored and/or lymphocytes will be obtained and transformed for later study.