GLYCOSIDASES IN HUMAN HEALTH AND DISEASE

糖苷酶在人类健康和疾病中的作用

• 批准号: 7955547
• 负责人: DAVID ROSE
• 金额: $ 4.32万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7955547
• 关键词: Alpha-glucosidase; Alpha-mannosidase; Complex; Computer Retrieval of Information on Scientific Projects Database; Data; Digestion; Disease; Enzymes; Funding; Glycoside Hydrolases; Golgi Apparatus; Grant; Health; Human; Institution; Intestines; Pathway interactions; Quality Control; Research; Research Personnel; Resolution; Resources; Source; Starch; Structure; United States National Institutes of Health; base; diabetic; glucosidase I; glycosylation; improved; inhibitor/antagonist; novel; protein folding

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We have been studying the structures and functions of glycoside hydrolases involved in human health and disease. Golgi alpha-mannosidase II is at the core of the mammalian N-glycosylation pathway, and is an anti-metastatic target. We plan to collect some complexes with novel inhibitors that have been developed based on our structural results (some of which were from CHESS data). Maltase glucoamylase is an important enzyme in intestinal starch digestion and a target for anti-diabetics. We solved the structure with CHESS data and are now collecting novel inhibitor complexes and attempting to improve the resolution. Finally, a-glucosidase I is the first enzyme in the protein folding `quality control` pathway. This is a new structure and we wil be collecting native data and possibly derivative (halide soak) data.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 我们一直在研究与人类健康和疾病有关的糖苷水解酶的结构和功能。高尔基体α-甘露糖苷酶II是哺乳动物N-糖基化途径的核心，并且是抗转移靶点。我们计划收集一些基于我们的结构结果（其中一些来自CHESS数据）开发的新型抑制剂的复合物。麦芽糖淀粉酶是肠道淀粉消化的重要酶，也是抗糖尿病药物的作用靶点。我们用CHESS数据解决了结构问题，现在正在收集新的抑制剂复合物，并试图提高分辨率。最后，α-葡糖苷酶I是蛋白质折叠“质量控制”途径中的第一个酶。这是一个新的结构，我们将收集原生数据和可能的衍生物（卤化物浸泡）数据。