FIBER DIFFRACTION FROM CELLULOSE IN GENETICALLY MODIFIED PLANTS

转基因植物中纤维素的纤维衍射

• 批准号: 7955582
• 负责人: DETLEF-M SMILGIES
• 金额: $ 1.17万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7955582
• 关键词: Area; Biology; Blood capillaries; Cell Wall; Cellulose; Collimator; Computer Retrieval of Information on Scientific Projects Database; Data; Development; Environment; Fiber; Funding; Genetically Modified Plants; Goals; Grant; Growth; Helium; Institution; Molecular; Noise; Plant Roots; Plants; Proteins; Research; Research Personnel; Resolution; Resources; Running; Signal Transduction; Source; Structure; Temperature; United States National Institutes of Health; capillary; cryogenics; detector; fascinate; research study; stem; success

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. A fascinating problem in biology is, how plants control the structure of their cell walls during growth on a molecular level. We propose to probe the cellulose structure in genetically modified plants, in plant parts such as stems or roots which grow significantly faster than in the wildtype. We obtained first results with wide-angle scattering/fiber diffraction on D-line in the last run, however, the data were limited by background levels and the limited detector area and resolution. At F1 we would like to use the helium environment developed for the protein microcrystallography effort, in combination with either a 100 micron collimator or a focusing capillary. Goal of this development study is to see, which features are essential to the success of this experiment where we would like to obtain as detailed data on the cellulose structure as possible (helium, prewetted helium at room temperature, dry helium for cryogenic temperature, detector range, signal-to-noise).

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 生物学中一个令人着迷的问题是，植物如何在分子水平上控制生长过程中的细胞壁结构。我们建议探索转基因植物中的纤维素结构，以及植物部分（例如茎或根）的纤维素结构，这些植物的生长速度明显快于野生型。在上次运行中，我们在 D 线上通过广角散射/光纤衍射获得了第一个结果，但是，数据受到背景水平以及有限的探测器面积和分辨率的限制。在 F1，我们希望使用为蛋白质微晶体学工作而开发的氦气环境，并结合 100 微米准直器或聚焦毛细管。 这项开发研究的目标是了解哪些特征对于本实验的成功至关重要，我们希望获得尽可能详细的纤维素结构数据（氦气、室温下预湿氦气、低温干燥氦气、探测器范围、信噪比）。