FIBER DIFFRACTION FROM CELLULOSE IN GENETICALLY MODIFIED PLANTS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 生物学中一个引人入胜的问题是，植物在生长过程中如何在分子水平上控制细胞壁的结构。我们建议探索转基因植物中的纤维素结构，在植物的茎或根等生长速度明显快于野生型的植物部分。在最后一次运行中，我们获得了D线上的广角散射/光纤衍射的初步结果，然而，数据受到背景水平和探测器面积和分辨率的限制。在F1，我们希望使用为蛋白质微晶学工作开发的氦环境，并结合100微米准直器或聚焦毛细管。这项发展研究的目标是了解哪些特征对于这项实验的成功至关重要，我们希望在其中获得关于纤维素结构的尽可能详细的数据(氦、室温下的预湿氦、低温下的干氦、探测器范围、信噪比)。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. A fascinating problem in biology is, how plants control the structure of their cell walls during growth on a molecular level. We propose to probe the cellulose structure in genetically modified plants, in plant parts such as stems or roots which grow significantly faster than in the wildtype. We obtained first results with wide-angle scattering/fiber diffraction on D-line in the last run, however, the data were limited by background levels and the limited detector area and resolution. At F1 we would like to use the helium environment developed for the protein microcrystallography effort, in combination with either a 100 micron collimator or a focusing capillary. Goal of this development study is to see, which features are essential to the success of this experiment where we would like to obtain as detailed data on the cellulose structure as possible (helium, prewetted helium at room temperature, dry helium for cryogenic temperature, detector range, signal-to-noise).