PHOSPHORYLATION REGULATES HUMAN CDC5 FUNCTION

磷酸化调节人类 CDC5 功能

• 批准号: 7957368
• 负责人: HAROLD S BERNSTEIN
• 金额: $ 0.01万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-06-01 至 2010-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7957368
• 关键词: Cells; Computer Retrieval of Information on Scientific Projects Database; Data; Funding; Grant; Human; Institution; Mammalian Cell; Maps; Mass Spectrum Analysis; Phosphorylation; Phosphorylation Site; Research; Research Personnel; Resources; Role; Serum; Source; Testing; United States National Institutes of Health; mutant

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Human CDC5 (hCDC5) is an important regulator of G2/M in mammalian cells. It is rapidly phosphorylated upon serum stimulation of cells, and we propose that phosphorylation regulates hCDC5 function. We seek to employ mass spectrometry to map the phosphorylation sites in hCDC5. We will use these data to make phosphospecific mutants and test their role in hCDC5 function.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 人CDC 5（hCDC 5）是哺乳动物细胞中G2/M期的重要调节因子。 它是快速磷酸化血清刺激后的细胞，我们建议磷酸化调节hCDC 5功能。 我们试图采用质谱法来绘制hCDC 5中的磷酸化位点。 我们将使用这些数据来制造磷酸特异性突变体并测试它们在hCDC 5功能中的作用。