TYPE 1 COPPER COORDINATION IN THE ABSENCE OF SULFUR

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 我们表达和纯化了铜绿假单胞菌天青素突变体，其还原电位和EPR谱类似于1型(T1)或蓝铜？尽管缺乏硫酸盐连接，但位点。到目前为止，研究表明硫代化合物对T1配位是必要的。与正在进行的电子吸收、EPR和电子转移动力学研究相结合，我们希望使用XAS和EXAFS分析来阐明我们特殊的EPR谱的来源，并评估氧化/还原过程中的位置重组。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We have expressed and purified mutants of Pseudomonas aeruginosa azurins which possess reduction potentials and EPR spectra akin to type 1 (T1) or ?blue copper? sites despite lack of thiolate ligation. To date research has indicated the necessity of thiolates for T1 coordination. In concert with ongoing electronic absorption, EPR, and electron transfer kinetics studies, we wish to use XAS and EXAFS analysis to elucidate the origin of our peculiar EPR spectra as well as to assess site reorganization during oxidation/reduction processes.