TYPE 1 COPPER COORDINATION IN THE ABSENCE OF SULFUR

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 我们表达和纯化的突变体铜绿假单胞菌天青蛋白具有还原电位和EPR谱类似于1型（T1）或？蓝铜？位点，尽管缺乏硫醇连接。迄今为止的研究表明，硫醇盐的必要性T1协调。与正在进行的电子吸收，EPR和电子转移动力学研究相一致，我们希望使用XAS和EXAFS分析来阐明我们特有的EPR光谱的起源，以及评估在氧化/还原过程中的网站重组。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We have expressed and purified mutants of Pseudomonas aeruginosa azurins which possess reduction potentials and EPR spectra akin to type 1 (T1) or ?blue copper? sites despite lack of thiolate ligation. To date research has indicated the necessity of thiolates for T1 coordination. In concert with ongoing electronic absorption, EPR, and electron transfer kinetics studies, we wish to use XAS and EXAFS analysis to elucidate the origin of our peculiar EPR spectra as well as to assess site reorganization during oxidation/reduction processes.