X-RAY DATA COLLECTION OF PROTEINS INVOLVED IN CELL ADHESION

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目及 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 细胞迁移和形态发生对于后生动物的发育、生长和存活是必不可少的，并且这些过程也在病理生理学场景中起作用，例如癌症转移和肌病。这些事件依赖于细胞吗？的能力，动态形成和打破特定的接触，杜撰的粘附连接，与它的邻居（adherens junctions）或细胞外基质（focal adhesions）。巨噬细胞蛋白是细胞-细胞（钙粘蛋白-连环蛋白介导的）和细胞-基质（整联蛋白-塔林蛋白介导的）连接的重要调节剂，其中它通过结合整合素复合物中的塔林蛋白或钙粘蛋白连接中的-连环蛋白和-辅肌动蛋白来提供与肌动蛋白细胞骨架的连接。为了了解粘附复合物中动力学的分子细节，我们将解决与细胞粘附复合物及其结合伴侣有关的几种蛋白质的晶体结构。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Cell migration and morphogenesis are essential for the development, growth, and survival of metazoans, and these processes are also at play in pathophysiological scenarios such as cancer metastasis and myopathies. These events rely on the cell?s ability to dynamically form and break specific contacts, coined adhesion junctions, with its neighbors (adherens junctions) or the extracellular matrix (focal adhesions). Vinculin is an essential regulator of both cell-cell (cadherin-catenin mediated) and cell-matrix (integrin-talin mediated) junctions, where it provides links to the actin cytoskeleton by binding to talin in integrin complexes, or to -catenin and -actinin in cadherin junctions. To understand the molecular details of the dynamics in adhesion complexes, we will solve the crystal structures of several proteins involved in cell adhesion in complex with their binding partners.