X-RAY DATA COLLECTION OF PROTEINS INVOLVED IN CELL ADHESION

细胞粘附相关蛋白质的 X 射线数据收集

• 批准号: 8362252
• 负责人: TINA IZARD
• 金额: $ 0.22万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2012-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362252
• 关键词: Actinin; Actins; Adherens Junction; Adhesions; Binding; Cadherins; Cell Adhesion; Cells; Coin; Complex; Cytoskeleton; Data Collection; Event; Extracellular Matrix; Focal Adhesions; Funding; Grant; Growth and Development function; Integrins; Link; Mediating; Molecular; Morphogenesis; Myopathy; National Center for Research Resources; Neoplasm Metastasis; Play; Principal Investigator; Process; Proteins; Radiation; Research; Research Infrastructure; Resources; Roentgen Rays; Source; Structure; Talin; United States National Institutes of Health; Vinculin; cell motility; cost; structural biology

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Cell migration and morphogenesis are essential for the development, growth, and survival of metazoans, and these processes are also at play in pathophysiological scenarios such as cancer metastasis and myopathies. These events rely on the cell?s ability to dynamically form and break specific contacts, coined adhesion junctions, with its neighbors (adherens junctions) or the extracellular matrix (focal adhesions). Vinculin is an essential regulator of both cell-cell (cadherin-catenin mediated) and cell-matrix (integrin-talin mediated) junctions, where it provides links to the actin cytoskeleton by binding to talin in integrin complexes, or to -catenin and -actinin in cadherin junctions. To understand the molecular details of the dynamics in adhesion complexes, we will solve the crystal structures of several proteins involved in cell adhesion in complex with their binding partners.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 细胞迁移和形态发生对于后生动物的发育，生长和存活至关重要，这些过程在诸如癌症转移和肌病等病理生理方面也起着作用。这些事件依赖于细胞的动态形成和破坏特定接触，固定的粘附连接，其邻居（粘附连接）或细胞外基质（焦点粘附）的能力。 Vinculin是细胞细胞（钙粘蛋白 - 钙蛋白介导的）和细胞矩阵（整联蛋白 - 核介导的）连接的必不可少的调节剂，它通过与整联蛋白络合物中的塔林结合，或与钙蛋白结合物中的塔林结合，或与钙蛋白蛋白结合的链球菌骨骼结合，在其中提供了与肌动蛋白细胞骨架的链接。为了了解粘附复合物中动力学的分子细节，我们将解决与其结合伴侣复合物中涉及细胞粘附的几种蛋白的晶体结构。