X-RAY DATA COLLECTION OF PROTEINS INVOLVED IN CELL ADHESION

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 细胞迁移和形态发生对后生动物的发育、生长和生存是必不可少的，这些过程也在癌症转移和肌病等病理生理场景中发挥作用。这些事件依赖于细胞？S与其邻居(粘连连接)或细胞外基质(焦点粘连)动态形成和断开特定接触的能力，称为粘连连接。纽蛋白是细胞-细胞(钙粘蛋白-连环蛋白介导的)和细胞-基质(整合素-连环蛋白介导的)连接的重要调节因子，它通过与整合素复合体中的Talin或钙粘蛋白连接中的-连环蛋白和-肌动蛋白连接来提供与肌动蛋白细胞骨架的连接。为了了解黏附复合体中动力学的分子细节，我们将解决参与细胞黏附的几种蛋白质与其结合伙伴的复合体的晶体结构。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Cell migration and morphogenesis are essential for the development, growth, and survival of metazoans, and these processes are also at play in pathophysiological scenarios such as cancer metastasis and myopathies. These events rely on the cell?s ability to dynamically form and break specific contacts, coined adhesion junctions, with its neighbors (adherens junctions) or the extracellular matrix (focal adhesions). Vinculin is an essential regulator of both cell-cell (cadherin-catenin mediated) and cell-matrix (integrin-talin mediated) junctions, where it provides links to the actin cytoskeleton by binding to talin in integrin complexes, or to -catenin and -actinin in cadherin junctions. To understand the molecular details of the dynamics in adhesion complexes, we will solve the crystal structures of several proteins involved in cell adhesion in complex with their binding partners.