FE(III) AND TRANSITION METALS TRANSPORT

FE(III) 和过渡金属传输

• 批准号: 7953870
• 负责人: Mark A Messerli
• 金额: $ 0.56万
• 依托单位: MARINE BIOLOGICAL LABORATORY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-12-01 至 2009-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7953870
• 关键词: Back; Cell Line; Cell membrane; Computer Retrieval of Information on Scientific Projects Database; Funding; Grant; Insecta; Institution; Iron; Lead; Measurement; Morphologic artifacts; Plants; Process; Research; Research Personnel; Resources; Soil; Source; System; Transition Elements; United States National Institutes of Health; Xenopus oocyte; extracellular; metal chelator; metal complex; stoichiometry; uptake

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Graminaceous plants take up Fe(III) and other transition metals from alkaline soils by secreting phytosiderophores, metal chelators, and then transporting the phytosiderophore-metal complex back across the plasma membrane with YS1 transporters, (Yellow-striped 1). Understanding more about this transporter could lead to an increased source of iron for the under-nourished, iron deficient world. Functional characterization has been performed in Xenopus oocytes. The electrochemical H+ gradient is thought to drive uptake of the phytosiderophore-metal complex. These results have recently been questioned due to the fact that the extracellular acidification used to drive uptake generates a well-characterized artifact in the Xenopus oocytes. We are in the process of expressing the transporters in other heterologous systems, less prone to this artifact, specifically mammalian and insect cell lines for the purpose of characterizing transport. Functional characterization in combination with electrochemical flux measurements will enable identification of the stoichiometry of the transporter.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 禾本科植物从碱性土壤中吸收Fe（III）和其他过渡金属，通过分泌植物铁载体、金属螯合剂，然后用YS 1转运蛋白将植物铁载体-金属复合物转运回质膜（黄色条纹1）。更多地了解这种转运蛋白可以为营养不良、缺铁的世界提供更多的铁来源。在非洲爪蟾卵母细胞中进行了功能表征。电化学H+梯度被认为驱动植物铁载体-金属络合物的吸收。 这些结果最近受到质疑，因为事实上，用于驱动摄取的细胞外酸化在非洲爪蟾卵母细胞中产生了一个很好的表征伪影。我们正在其他异源系统中表达转运蛋白的过程中，不太容易出现这种人工制品，特别是哺乳动物和昆虫细胞系，目的是表征运输。结合电化学通量测量的功能表征将能够识别转运蛋白的化学计量。