CATION-PROTON ANTIPORTERS (CPAS): A NEW GROUP OF PH REGULATING TRANSPORTERS

阳离子-质子反转运蛋白 (CPAS)：一组新的 PH 调节转运蛋白

• 批准号: 7953869
• 负责人: Mark A Messerli
• 金额: $ 0.56万
• 依托单位: MARINE BIOLOGICAL LABORATORY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-12-01 至 2009-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7953869
• 关键词: Cations; Cell membrane; Cells; Computer Retrieval of Information on Scientific Projects Database; Culicidae; Funding; Gastrointestinal tract structure; Grant; Institution; Ions; Measurement; Oocytes; Protons; Research; Research Personnel; Resources; Sequence Homology; Source; System; United States National Institutes of Health; Xenopus oocyte; antiporter

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Cation-Proton Antiporters (CPAs) are a new group of pH regulating transporter that have recently been cloned and localized to specific regions of the mosquito alimentary canal. No functional characterization has been performed for these mosquito transporters and their function has only been inferred from sequence homology, 2H+ are thought to enter the cell in exchange for efflux of 1 Na+. One of the cloned sequences, AgNHA1, has been setup to be expressed in the Xenopus oocyte heterologous expression system. As the ions are exiting the oocytes it is very difficult to identify exactly which cations are passing out through the plasma membrane. The current hypothesis is that it could be either Na+ or K+. We have performed preliminary flux measurements for Na+ in support of a grant submission.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 阳离子质子反向转运蛋白（CPAs）是近年来克隆的一类新的pH调节转运蛋白，定位于蚊子消化道的特定区域。尚未对这些蚊子转运蛋白进行功能表征，仅从序列同源性推断其功能，认为2 H+进入细胞以交换1 Na+的流出。克隆的序列之一，AgNHA 1，已被设置为在非洲爪蟾卵母细胞异源表达系统中表达。 当离子离开卵母细胞时，很难准确地识别哪些阳离子穿过质膜。目前的假设是，它可能是Na+或K+。我们已经进行了初步的Na+通量测量，以支持资助申请。