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DIGITAL IMAGING OF FIREFLY LUCIFERASE ACTIVITY IN MOUSE EMBRYONIC STEM CELLS

DIGITAL IMAGING OF FIREFLY LUCIFERASE ACTIVITY IN MOUSE EMBRYONIC STEM CELLS
小鼠胚胎干细胞中萤火虫荧光素酶活性的数字成像
批准号：
7956509
负责人：
Ken W.Y. Cho
金额：
$0.53万
依托单位：
UNIVERSITY OF CALIFORNIA-IRVINE
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
项目状态：
已结题
起止时间：
2009-08-01 至 2010-07-31
来源：
https://reporter.nih.gov/project-details/7956509
关键词：
Computer Retrieval of Information on Scientific Projects DatabaseES Cell LineFirefly LuciferasesFluorescenceFundingGrantInstitutionLaboratoriesLight ExerciseLuciferasesMusPlasmidsReporterResearchResearch PersonnelResourcesSourceStem cellsUnited States National Institutes of Healthcharge coupled device cameradigital imagingembryonic stem cell

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中文摘要
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英文摘要
This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We are transfecting CMV-Luciferase plasmid into mouse embryonic stem cells and then viewing luciferase activity via a CCD camera to capture light activity. We are also generating a stable BMP responsive luciferase reporter mouse embryonic stem cell line and look at luciferase activity of our stem cells.
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