Assessment of the phasor Fluorescence Lifetime Imaging Microscopy (FLIM) Approach in an animal model
Assessment of the phasor Fluorescence Lifetime Imaging Microscopy (FLIM) Approach in an animal model
批准号:
9396700
负责人:
Ken W.Y. Cho
金额:
$22.19万
依托单位国家:
美国
项目类别:
财政年份:
2017
资助国家:
美国
项目状态:
已结题
起止时间:
2017-08-01 至 2019-07-31
关键词:
AdoptedAffectAlgorithmsAnimal ModelAnimalsAssisted Reproductive TechnologyBehaviorBiological AssayBirthBreedingCell CountCell SizeCellsCessation of lifeCharacteristicsChemicalsClassificationClinicComplexCulture MediaCulture TechniquesDataDetectionDevelopmentDevelopment PlansEmbryoEmbryo TransferEmbryonic DevelopmentEmbryonic Lethal MutationEnergy-Generating ResourcesEvaluationFertilization in VitroFutureGenerationsGeneticGenomicsGlucoseGuidelinesHumanHuman ResourcesIn VitroIndividualLive BirthMaintenanceManualsMeasurementMeasuresMetabolicMicroscopicMorphologyMultiple PregnancyMusNoiseNutrientOocytesOvulation InductionOxygenPerformancePhasePhysical shapePhysiciansPregnancyPremature BirthProceduresProcessProteomicsPyruvateReactive Oxygen SpeciesReadingRetrievalRiskS PhaseSafetySignal TransductionSolidSpectroscopy, Fourier Transform InfraredSystemTechniquesTechnologyTestingTimeTrainingWomanWorkassisted reproductionbaseblastocystdisabilityfluorescence lifetime imagingimaging approachimplantationimprovedindexinginstrumentationmutantnatural Blastocyst Implantationneonatenovel strategiesoxidized lipidpreimplantationreproductivesuccess
中文摘要
摘要
英文摘要
Abstract
The latest figure is that around 1.5 million Assisted Reproductive Technology (ART) cycles are performed
each year worldwide, with an estimated 350,000 babies born. One of the critical steps during the IVF
process is the selection of high-quality embryos for uterine transfer. This selection is currently based largely
on defined morphological criteria and physical characteristics of the blastocyst. While such criteria have
proven to be useful in improving implantation rates, assessment of the reproductive potential of individual
embryos is not sufficient. Therefore, IVF centers often perform simultaneous transfers of multiple embryos
that can result in multiple pregnancies, thus increasing the risk of preterm delivery and the death or lifelong
disability of neonates. As the number of assisted reproduction cycles worldwide is increasing, improvements
in our ability to predict embryo viability is urgently needed. Development of more qualitative and objective
means for assessing embryo quality and viability that are safer and faster could provide significant
advances in IVF by enabling singleton embryo transfers rather than the implantation of multiple embryos in
order to increase the likelihood of a successful pregnancy. Given the limitations of morphologic evaluation,
several technologies have been explored for the assessment of embryo viability. These include the
measurement of metabolites in embryonic culture media along with genomic and proteomic profiling of the
embryos themselves. Spectroscopic approaches have also been utilized to measure the amount of
metabolites that arise during pre-implantation development. However, these approaches are time-
consuming and require highly-trained personnel to analyze the complex data. Here we describe the
application of a phasor-FLIM (Fluorescence-Lifetime Imaging Microscopy) approach, which is a “non-
invasive” live imaging approach capable of measuring endogenous autofluorescent metabolites within living
embryos undergoing in vitro culturing. The approach captures information on the metabolic energy sources
utilized by pre-implantation embryos as readout of embryo quality and viability.
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海外基金