HEPARIN STRUCTURE STUDY

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 肝素因其抗凝血活性而广为人知，因为它能够加速抗凝血酶抑制血液凝固级联中丝氨酸蛋白酶的速度。肝素是一种线性聚合物，由1？4连接的吡喃糖醛酸和氨基葡萄糖残基的重复单元组成，含有许多给予它高负电荷的磺基。核磁共振是一种强大的技术，能够研究糖胺多糖的结构，而不需要事先通过化学或酶作用将其裂解成较小的寡糖。因此，核磁共振可以用来避免当多糖被分解成较小的寡糖时导致的结构信息的损失。在这里，我们用质子和碳13核磁共振研究了肝素的结构和不同制备批次之间的差异。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Heparin is well-known for its anticoagulant activity based on its ability to accelerate the rate at which antithrombin inhibits serine proteases in the blood coagulation cascade. Heparin is a linear polymer consisting of repeating units of 1¿4 linked pyranosyluronic acid and glucosamine residues and contains many sulfo groups that give it a high negative charge. NMR is a powerful technique capable of studying the structure of glycosaminoglycans without prior chemical or enzymatic cleavage into smaller oligosaccharides. Thus, NMR can be used to avoid the loss of structural information that results when the polysaccharides are broken down to smaller oligosaccharides. Here we performed proton and carbon 13 NMR to study the structure of heparin and the difference among different preparative lots.