HEPARIN STRUCTURE STUDY

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 肝素因其抗凝活性而闻名，这是基于其加速抗凝血酶抑制血液凝固级联中丝氨酸蛋白酶的速率的能力。肝素是一种线性聚合物，由1 <$4连接的吡喃糖醛酸和葡萄糖胺残基的重复单元组成，含有许多磺基，使其具有高负电荷。NMR是一种强大的技术，能够研究糖胺聚糖的结构，而无需事先化学或酶裂解成较小的寡糖。因此，NMR可用于避免当多糖被分解成较小的寡糖时导致的结构信息的损失。本文通过核磁共振氢谱和碳13谱研究了肝素的结构以及不同制备批次之间的差异。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Heparin is well-known for its anticoagulant activity based on its ability to accelerate the rate at which antithrombin inhibits serine proteases in the blood coagulation cascade. Heparin is a linear polymer consisting of repeating units of 1¿4 linked pyranosyluronic acid and glucosamine residues and contains many sulfo groups that give it a high negative charge. NMR is a powerful technique capable of studying the structure of glycosaminoglycans without prior chemical or enzymatic cleavage into smaller oligosaccharides. Thus, NMR can be used to avoid the loss of structural information that results when the polysaccharides are broken down to smaller oligosaccharides. Here we performed proton and carbon 13 NMR to study the structure of heparin and the difference among different preparative lots.