HEPARIN STRUCTURE STUDY

肝素结构研究

• 批准号: 8361166
• 负责人: JIN P XIE
• 金额: $ 0.11万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2012-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361166
• 关键词: Acids; Anticoagulants; Antithrombins; Blood coagulation; Carbon; Charge; Chemicals; Funding; Glucosamine; Glycosaminoglycans; Grant; Heparin; Link; National Center for Research Resources; Oligosaccharides; Polymers; Polysaccharides; Principal Investigator; Protons; Research; Research Infrastructure; Resources; Serine Protease; Source; Structure; Techniques; United States National Institutes of Health; base; cost

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Heparin is well-known for its anticoagulant activity based on its ability to accelerate the rate at which antithrombin inhibits serine proteases in the blood coagulation cascade. Heparin is a linear polymer consisting of repeating units of 1¿4 linked pyranosyluronic acid and glucosamine residues and contains many sulfo groups that give it a high negative charge. NMR is a powerful technique capable of studying the structure of glycosaminoglycans without prior chemical or enzymatic cleavage into smaller oligosaccharides. Thus, NMR can be used to avoid the loss of structural information that results when the polysaccharides are broken down to smaller oligosaccharides. Here we performed proton and carbon 13 NMR to study the structure of heparin and the difference among different preparative lots.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 肝素因其抗凝活性而闻名，这是基于其加速抗凝血酶抑制血液凝固级联中丝氨酸蛋白酶的速率的能力。肝素是一种线性聚合物，由1 <$4连接的吡喃糖醛酸和葡萄糖胺残基的重复单元组成，含有许多磺基，使其具有高负电荷。NMR是一种强大的技术，能够研究糖胺聚糖的结构，而无需事先化学或酶裂解成较小的寡糖。因此，NMR可用于避免当多糖被分解成较小的寡糖时导致的结构信息的损失。本文通过核磁共振氢谱和碳13谱研究了肝素的结构以及不同制备批次之间的差异。