FRET ANALYSIS OF FISSION YEAST SPB COMPONENTS

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 为了进一步了解裂殖酵母SPB，必须确定其组织。 从Ppc89开始，我们计划建立一个微管组织中心是如何组装的图片。 我们已经有证据表明Ppc89和另一种参与组装信号级联反应的SPB蛋白Sid4之间存在直接相互作用，现在计划使用这两种蛋白质作为顶点与其他FRET伙伴组一起向前推进。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In order to gain further understanding of the fission yeast SPB, its organization must be determined. Beginning with Ppc89, we plan to build a picture of how this microtubule organizing center is assembled. We already have evidence for direct interaction between Ppc89 and another SPB protein involved in assembling a signaling cascade, Sid4, and now plan to move forward with other sets of FRET partners using these two proteins as vertices.