FRET ANALYSIS OF FISSION YEAST SPB COMPONENTS

裂殖酵母 SPB 成分的 FRET 分析

• 批准号: 7957792
• 负责人: Kathleen L Gould
• 金额: $ 1.63万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-01 至 2010-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7957792
• 关键词: Biology; Computer Retrieval of Information on Scientific Projects Database; Fission Yeast; Fluorescence Resonance Energy Transfer; Funding; Fungal Genome; Grant; Institution; Microtubule-Organizing Center; Proteins; Research; Research Personnel; Resources; Signal Transduction; Source; United States National Institutes of Health

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In order to gain further understanding of the fission yeast SPB, its organization must be determined. Beginning with Ppc89, we plan to build a picture of how this microtubule organizing center is assembled. We already have evidence for direct interaction between Ppc89 and another SPB protein involved in assembling a signaling cascade, Sid4, and now plan to move forward with other sets of FRET partners using these two proteins as vertices.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 为了进一步了解裂殖酵母SPB，必须确定其组织。 从Ppc 89开始，我们计划建立一个微管组织中心是如何组装的图片。 我们已经有证据表明Ppc 89和另一种参与组装信号级联反应的SPB蛋白Sid 4之间存在直接相互作用，现在计划使用这两种蛋白质作为顶点与其他FRET伙伴组一起向前推进。