IDENTIFICATION OF FAB-7 BOUNDARY PROTEINS

FAB-7 边界蛋白的鉴定

• 批准号: 7957799
• 负责人: Paul D Schedl
• 金额: $ 0.33万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-01 至 2010-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7957799
• 关键词: Adult; Binding; Biology; Chromatin; Cis-Acting Sequence; Complex; Computer Retrieval of Information on Scientific Projects Database; DNA Sequence; Development; Distal; Drosophila genus; Elements; Embryo; Embryonic Development; Ensure; Eukaryota; Funding; Fungal Genome; Grant; Institution; Proteins; Research; Research Personnel; Resources; Side; Site; Source; Staging; Tissues; Trans-Activators; United States National Institutes of Health; fly; nuclease; protein complex

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Fab-7 boundary functions to ensure the autonomous activity of the iab-6 and iab-7 cis-regulatory domains in the Drosophila Bithorax Complex from early embryogenesis through to the adult stage. Although Fab-7 is only required for the proper development of a single posterior parasegment, it is active in all tissues and stages of development that have been examined. In this respect Fab-7 resembles conventional constitutive boundaries in flies and other eukaryotes that act through ubiquitous cis-elements and trans-acting factors. The Fab-7 boundary spans a DNA sequence of ~1.2 kb and contains 3 chromatin specific nuclease hypersensitive sites. We have examined the functioning of cis-acting sequences in the largest of these hypersensitive sites, HS1, in detail. Though Fab-7 boundary activity is constitutive, we found that HS1 is divided into sub-elements whose boundary activity is restricted to only a specific stage in development. One sub-element, which is located on the proximal side of HS1, is active only in the early embryo. A second sub-element, which is located in the middle of HS1, is not active in early embryos; however, it becomes active mid-way through embryogenesis and then remains active through to the adult stage. A third sub-element on the distal side of HS1 confers boundary activity from early through mid-embryogenesis but not later. Through this project, we identified candidate proteins that are likely to confer this early-stage specific boundary activity, and then found that these proteins make a heterogeneous protein complex to bind the target sequence. We also would like to identify the trans-acting factors that confer other stage specific boundary activity in each.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 Fab-7边界的功能是确保果蝇双胸复合体中iab-6和iab-7顺式调节结构域从早期胚胎发生到成年阶段的自主活性。 虽然Fab-7仅为单个后旁节的正常发育所需，但它在已检查的所有组织和发育阶段中均具有活性。 在这方面，Fab-7类似于苍蝇和其他真核生物中通过普遍存在的顺式元件和反式作用因子起作用的常规组成性边界。 Fab-7边界跨越约1.2kb的DNA序列，并含有3个染色质特异性核酸酶超敏位点。 我们已经研究了最大的这些过敏网站，HS 1，详细的顺式作用序列的功能。 尽管Fab-7的边界活性是组成性的，但我们发现HS 1被分成子元件，其边界活性仅限于发育的特定阶段。 一个位于HS 1近端的子元件仅在早期胚胎中活跃。 位于HS 1中间的第二个子元件在早期胚胎中不活跃;然而，它在胚胎发生的中途变得活跃，然后保持活跃直到成体阶段。 HS 1远端的第三个子元件赋予从早期到中期胚胎发生的边界活动，但不晚。 通过这个项目，我们鉴定了可能赋予这种早期特异性边界活性的候选蛋白质，然后发现这些蛋白质形成异质蛋白质复合物来结合靶序列。 我们还想确定在每个阶段赋予其他特定边界活动的反作用因素。