CONDENSIN COMPLEXES IN C ELEGANS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 凝聚素复合物在有丝分裂期间起作用以形成凝聚的有丝分裂染色体，并且在间期期间起作用以调节基因的表达。这两个目标都被认为是通过改变染色体的高级结构来实现的。在线虫中，C.两种不同的凝聚素复合物执行这两种功能。有丝分裂凝聚素复合体在染色体分离中起作用，而剂量补偿复合体调节两性X染色体上的基因表达，以平衡两性之间的X连锁基因剂量。我们的长期目标是破译蠕虫剂量补偿的分子机制，并确定它如何与染色体凝聚的机制。剂量补偿复合物的生物化学纯化导致CAPG-1的发现，CAPG-1是一种新的剂量补偿复合物亚基，也在染色体分离中起作用。为了揭示剂量补偿和染色体分离之间的机制相似性，我们将使用遗传，生物化学和显微镜工具来分析CAPG-1在这两个过程中发挥作用的方式。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The condensin complex functions both during mitosis, to form condensed mitotic chromosomes, and during interphase, to regulate expression of genes. Both of these goals are thought to be accomplished by altering the higher order of structure of chromosomes. In the nematode, C. elegans, two distinct condensin complexes perform these two functions. The mitotic condensin complex plays a role in chromosome segregation, while the dosage compensation complex regulates expression of genes on hermaphrodite X chromosomes to equalize X-linked gene dosage between the sexes. Our long-term goal is to decipher the molecular mechanism of worm dosage compensation and to determine how it relates to the mechanism of chromosome condensation. Biochemical purification of the dosage compensation complex led to the discovery of CAPG-1, a new dosage compensation complex subunit, which also functions in chromosome segregation. To reveal mechanistic similarities between dosage compensation and chromosome segregation, we will use genetic, biochemical, and microscopic tools to analyze the ways in which CAPG-1 can function during both processes.