CONDENSIN COMPLEXES IN C ELEGANS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 凝集素复合体在有丝分裂过程中发挥作用，形成浓缩的有丝分裂染色体，并在间期调节基因的表达。这两个目标都被认为是通过改变染色体的更高顺序结构来实现的。在线虫中，两个不同的凝集素复合体执行这两个功能。有丝分裂凝集素复合体在染色体分离中起作用，而剂量补偿复合体调节两性X染色体上的基因表达，使X连锁基因在两性之间的剂量相等。我们的长期目标是破译蠕虫剂量补偿的分子机制，并确定它与染色体凝聚机制的关系。剂量补偿复合体的生化纯化导致了CAPG-1的发现，CAPG-1是一种新的剂量补偿复合体亚基，它也在染色体分离中发挥作用。为了揭示剂量补偿和染色体分离之间的机制相似性，我们将使用遗传学、生化和显微工具来分析CAPG-1在这两个过程中发挥作用的方式。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The condensin complex functions both during mitosis, to form condensed mitotic chromosomes, and during interphase, to regulate expression of genes. Both of these goals are thought to be accomplished by altering the higher order of structure of chromosomes. In the nematode, C. elegans, two distinct condensin complexes perform these two functions. The mitotic condensin complex plays a role in chromosome segregation, while the dosage compensation complex regulates expression of genes on hermaphrodite X chromosomes to equalize X-linked gene dosage between the sexes. Our long-term goal is to decipher the molecular mechanism of worm dosage compensation and to determine how it relates to the mechanism of chromosome condensation. Biochemical purification of the dosage compensation complex led to the discovery of CAPG-1, a new dosage compensation complex subunit, which also functions in chromosome segregation. To reveal mechanistic similarities between dosage compensation and chromosome segregation, we will use genetic, biochemical, and microscopic tools to analyze the ways in which CAPG-1 can function during both processes.