CONDENSIN COMPLEXES IN C ELEGANS

线虫中的凝结蛋白复合物

• 批准号: 7957810
• 负责人: Gyorgyi Csankovszki
• 金额: $ 0.33万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-01 至 2010-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7957810
• 关键词: Biochemical; Biology; Caenorhabditis elegans; Chromosome Condensation; Chromosome Segregation; Chromosomes; Complex; Computer Retrieval of Information on Scientific Projects Database; Dosage Compensation (Genetics); Funding; Fungal Genome; Gene Dosage; Gene Expression; Genetic; Goals; Grant; Higher Order Chromatin Structure; Institution; Interphase; Link; Microscopic; Mitosis; Mitotic; Mitotic Chromosome; Molecular; Nematoda; Play; Process; Research; Research Personnel; Resources; Role; Source; United States National Institutes of Health; X Chromosome; condensin; sex; tool

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The condensin complex functions both during mitosis, to form condensed mitotic chromosomes, and during interphase, to regulate expression of genes. Both of these goals are thought to be accomplished by altering the higher order of structure of chromosomes. In the nematode, C. elegans, two distinct condensin complexes perform these two functions. The mitotic condensin complex plays a role in chromosome segregation, while the dosage compensation complex regulates expression of genes on hermaphrodite X chromosomes to equalize X-linked gene dosage between the sexes. Our long-term goal is to decipher the molecular mechanism of worm dosage compensation and to determine how it relates to the mechanism of chromosome condensation. Biochemical purification of the dosage compensation complex led to the discovery of CAPG-1, a new dosage compensation complex subunit, which also functions in chromosome segregation. To reveal mechanistic similarities between dosage compensation and chromosome segregation, we will use genetic, biochemical, and microscopic tools to analyze the ways in which CAPG-1 can function during both processes.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 凝聚蛋白复合物在有丝分裂期间发挥作用，形成浓缩的有丝分裂染色体，并在间期期间发挥作用，调节基因的表达。这两个目标都被认为是通过改变染色体的高级结构来实现的。在线虫中，两种不同的凝缩蛋白复合物执行这两种功能。有丝分裂凝聚蛋白复合物在染色体分离中发挥作用，而剂量补偿复合物调节雌雄同体X染色体上的基因表达，以平衡两性之间的X连锁基因剂量。我们的长期目标是破译蠕虫剂量补偿的分子机制，并确定它与染色体浓缩机制的关系。剂量补偿复合物的生化纯化导致了 CAPG-1 的发现，CAPG-1 是一种新的剂量补偿复合物亚基，它也在染色体分离中发挥作用。为了揭示剂量补偿和染色体分离之间的机制相似性，我们将使用遗传、生化和微观工具来分析 CAPG-1 在这两个过程中发挥作用的方式。