CONDENSIN COMPLEXES IN C ELEGANS

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This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Condensin complexes are essential for mitotic chromosome condensation in all eukaryotes. C. elegans possesses two condensin complexes, one is involved in mitosis, and the other, the DCC, binds both hermaphrodite X-chromosomes to repress gene expression by half during dosage compensation. We recently identified a new subunit of the DCC, Cel-CAPG-1, a homolog of condensin subunit CAP-G. We found that in addition to interacting with the DCC, CAPG-1 also associates with mitotic condensin subunits. In addition, capg-1 mutants exhibit both dosage compensation and chromosome segregation defects. The goal of this project is to find CAPG-1 interacting proteins both during dosage compensation, and during mitosis. We are purifying CAPG-1 containing complexes from C. elegans embryos and adults to compare the list of interacting partners during various processes.