MODELING OF VIRAL PACKAGING MOTORS

病毒包装电机的建模

• 批准号: 7957349
• 负责人: CHARLES L BROOKS
• 金额: $ 1.83万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-01 至 2010-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7957349
• 关键词: Adenosine Triphosphate; Bacteriophages; Behavior; Capsid; Cereals; Computer Retrieval of Information on Scientific Projects Database; Cryoelectron Microscopy; DNA; Funding; Grant; Hydrolysis; Institution; Modeling; Molecular; Molecular Motors; Motor; Nucleic Acids; Research; Research Personnel; Resolution; Resources; Source; Structure; United States National Institutes of Health; Viral Packaging; Virus; X-Ray Crystallography; insight; multi-scale modeling; simulation; structural biology; tool

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Many viruses use molecular motors to package the nucleic acid into capsids. The viral packaging motor is ahighly robust and efficient molecular machinery driven by the adenosine triphosphate (ATP) hydrolysis. The high-resolution structure of the packaging motor was solved for the bacteriophage phi29 and phi22 by means of X-ray crystallography and cryo-electron microscopy. Due to the complexity of its structure, however, the dynamic behavior of the packaging motor still remains elusive. Understanding the dynamic mechanism of the packaging motor is essential to provide new insights into the principles of molecular machinery. In this study, we will investigate the DNA dynamics in the packaging motor using coarse-grained and detailed atomistic simulations.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 许多病毒使用分子电动机将核酸包装到衣壳中。由三磷酸腺苷（ATP）水解驱动的病毒包装电动机非常可增强，有效的分子机械。通过X射线晶体学和冷冻电子显微镜为噬菌体PHI29和PHI22求解包装电机的高分辨率结构。然而，由于其结构的复杂性，包装电机的动态行为仍然难以捉摸。了解包装电机的动态机制对于提供分子机械原理的新见解至关重要。在这项研究中，我们将使用粗粒和详细的原子模拟研究包装电机中的DNA动力学。