HISTONE METHYLATION AND EPIGENETIC CONTROL OF THE GENOME

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目及 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 组蛋白的残基特异性甲基化导致不同的表观遗传状态。但是组蛋白甲基转移酶（将甲基添加到组蛋白的酶）和组蛋白去甲基化酶（从组蛋白中去除甲基的酶）如何靶向特定的染色体结构域还没有很好的理解。更重要的是，除了几个众所周知的例子，细胞机制如何识别这些修饰尚不清楚。我们将从分裂酵母中纯化组蛋白甲基转移酶和脱甲基酶复合物，以鉴定调节组蛋白甲基化的新蛋白。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Residue specific methylation of histones results in diverse epigenetic states. But how histone methyltransferases (enzymes that add methyl group to histones) and histone demethylases (enzymes that remove methyl group from histones) are targeted to specific chromosome domains are not well understood. More importantly, apart from several well-known examples, how the cellular machinery recognizes these modifications is not clear. We will purify histone methyltransferase and demethylases complexes from fission yeast to identify novel proteins that regulate histone methylation.