STRUCTURE DETERMINATION OF HALOGENASE CMLS

卤素酶 CMLS 的结构测定

• 批准号: 7957234
• 负责人: ZONGCHAO JIA
• 金额: $ 0.92万
• 依托单位: BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7957234
• 关键词: Active Sites; Anabolism; Antibiotics; Computer Retrieval of Information on Scientific Projects Database; Crystallography; Flavins; Funding; Grant; Institution; Lead; Light; Operative Surgical Procedures; Research; Research Personnel; Resources; Source; Structure; Substrate Specificity; Synchrotrons; United States National Institutes of Health; drug candidate; insight; novel

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. CmlS is a flavin-dependent halogenase believed to take part in the biosynthesis of chloramphenical. CmlS is thought to dichlorinate the acetyl moiety of the antibiotic although nothing to date is known about it's mechanism of operation or substrate specificity. CmlS is novel in that it is one of few flavin-dependent halogenases believed to act upon a aliphatic rather than aromatic substrate. By crystallizing CmlS there is hope to gain information as to its active site and hopefully gain insight into what substates it may act upon. Successful elucidation of CmlS' activity may lead to furthering antibiotic research and hopefully produce new drug candidates.

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 CMLS是一种依赖黄素的卤化酶，被认为参与了氯霉素的生物合成。CMLS被认为可以使抗生素的乙酰基部分二氯化，尽管到目前为止还不知道它的操作机制或底物专一性。CMLS是新奇的，因为它是为数不多的依赖黄素的卤代酶之一，被认为作用于脂肪族而不是芳香族底物。通过结晶CML，有希望获得关于其活性部位的信息，并有望获得它可能作用于哪些亚态的洞察。对CMLS活性的成功阐明可能会导致进一步的抗生素研究，并有望产生新的候选药物。