STRUCTURAL DETERMINATIONS OF PROLAMINES FROM RICE

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 醇溶谷蛋白是在种子中发现的主要类型的储存蛋白之一，因此是来自谷物的蛋白质的膳食来源。醇溶谷蛋白保留在ER腔中，并组装成脑池内包涵体颗粒。它们富含脯氨酸和谷氨酰胺。贮藏蛋白是植物胚胎发育的氮源，也在人类营养中发挥作用。储存蛋白可能与食物过敏有关。 一些植物中的醇溶谷蛋白与乳糜泻有关。虽然在水稻中并非如此，但在这种生物体中也有必要进行彻底的研究。几种醇溶谷蛋白已被纯化，其结构正在确定。正在研究的一个方面是糖基化的可能发生。采用高效液相色谱法分离了稻米醇溶蛋白，并对两个主要组分进行了质谱分析。 在这两个馏分中，一个主要的组成部分，可以表征和部分测序的MALDI-TOF MS，并通过串联nanospray MS的三重四极杆，QoTOF，和FT-ICR质谱仪。蛋白质印迹和酶分析提供了一些证据，证明混合物中的一种组分具有O-连接修饰，包括O-GlcNAc。描述该结果的手稿最近发表在《植物生理学杂志》上。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Prolamines are one of the major types of storage proteins found in seeds and thus are a dietary source of protein from grains. The prolamines are retained in the ER lumen and are assembled into intracisternal inclusion granules. They are rich in proline and glutamine. The storage proteins serve as a nitrogen source for the developing plant embryo and also play a role in human nutrition. It is possible that the storage proteins may be implicated in food allergies. Prolamines in some plants are involved in Celiac Disease. While this is not the case in rice, their thorough investigation is also warranted in this organism. Several prolamines from rice have been purified and their structures are being determined. One aspect being investigated is the possible occurrence of of glycosylation. The prolamines from rice were separated by HPLC, and the two major fractions have been investigated by mass spectrometry. In both fractions, a major component could be characterized and partally sequenced by MALDI-TOF MS, and by tandem nanospray MS on triple quadrupole, QoTOF, and FT-ICR mass spectrometers. Western blots and enzyme analyses have provided some evidence that a component in the mixture has O-linked modifications, including O-GlcNAc. A manuscript describing the results was recently published in the Journal of Plant Physiology.