STRUCTURAL DETERMINATIONS OF PROLAMINES FROM RICE

大米中谷醇溶蛋白的结构测定

• 批准号: 7602012
• 负责人: Lokesh Joshi
• 金额: $ 0.65万
• 依托单位: BOSTON UNIVERSITY MEDICAL CAMPUS
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-03 至 2008-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7602012
• 关键词: Celiac Disease; Cereals; Computer Retrieval of Information on Scientific Projects Database; Cytoplasmic Granules; Food Hypersensitivity; Funding; Glutamine; Grant; High Pressure Liquid Chromatography; Human; Institution; Investigation; Link; Mass Spectrum Analysis; Minor; Modification; Nitrogen; Organism; Plants; Play; Prolamine; Proline; Proteins; Research; Research Personnel; Resources; Rice; Role; Seeds; Source; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Structure; United States National Institutes of Health; Western Blotting; glycosylation; mass spectrometer; nutrition

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Prolamines are one of the major types of storage proteins found in seeds and thus are a dietary source of protein from grains. The prolamines are retained in the ER lumen and are assembled into intracisternal inclusion granules. They are rich in proline and glutamine. The storage proteins serve as a nitrogen source for the developing plant embryo and also play a role in human nutrition. It is possible that the storage proteins may be implicated in food allergies. Prolamines in some plants are involved in Celiac Disease. While this is not the case in rice, their thorough investigation is also warranted in this organism. Several prolamines from rice have been purified and their structures are being determined. One aspect being investigated is the possibe occurrence of of glycosylation. The prolamines from rice were separated by HPLC, and the two major fractions have been investigated by mass spectrometry. In both fractions, a major component could be characterized and partally sequenced by MALDI TOF MS, and by tandem nanospray MS on triple quadrupole, QoTOF, and FT-ICR mass spectrometers. Further analysis by FT-ICR MS should also allow for the characterization of the minor components. Western blots have recently provided some evidence that a component in the mixture has O-linked modifications, including possibly O-GlcNAc..

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 醇溶蛋白是种子中发现的主要储存蛋白之一，因此是谷物蛋白质的饮食来源。醇溶蛋白保留在内质网管腔内，并组装成脑池内包涵体颗粒。它们富含脯氨酸和谷氨酰胺。这些贮藏蛋白为发育中的植物胚胎提供氮源，也在人体营养中发挥作用。储存蛋白可能与食物过敏有关。一些植物中的醇溶蛋白与腹水症有关。虽然大米的情况并非如此，但他们对这种有机体的彻底研究也是有必要的。大米中的几种醇溶蛋白已被提纯，其结构正在确定中。正在研究的一个方面是糖基化的可能发生。用高效液相色谱分离了大米中的醇溶蛋白，并对其两个主要组分进行了质谱分析。在这两个组分中，一个主要组分可以用MALDI TOF MS进行表征和部分测序，而在三个四极杆、QOTOF和FT-ICR质谱仪上可以用串联纳米质谱仪进行表征和部分测序。FT-ICR MS的进一步分析还应允许对次要成分进行表征。最近，Western blotts提供了一些证据，表明混合物中的一个成分具有O-连接的修饰，可能包括O-GlcNAc。