Sequencing of EST libraries from the European eel (Anguilla anguilla)

摘要：水生生物在许多不同的淡水（FW）和海水（SW）栖息地的进化成功，是由于复杂的相互关联的离子和水运输系统的发展，使它们能够在极端低渗和高渗环境中渗透调节和生存。这方面的研究很充分的例子是广盐硬骨鱼，如欧洲鳗鱼，它们表现出遗传可塑性，使它们能够在FW和SW环境中生存，而体液的渗透压和离子组成只有很小的变化。欧洲鳗鱼已被证明是研究广盐硬鱼渗透调节的优秀模型物种，最近，由NERC资助的工作已经能够从鳃、肠、肾、性腺和脑中产生EST文库，这些文库总共包含超过12,000个组织特异性克隆。该鳗鱼克隆装置已用于生产两个独立的18,000个特征微阵列，这些微阵列已生产出来，目前可供利物浦大学生物科学学院利物浦微阵列设施使用。然而，只有不到1200个克隆作为阵列的特征被测序。此应用程序要求支持对剩余克隆进行排序，并对库进行一般管理，这将涉及重新封装所有克隆，以将冗余减少到5%以下。重新制定的文库将完全注释，est将根据序列同源性与其他硬骨鱼基因组（斑马鱼，河豚）和其他基因库中存放的基因进行表征。序列信息不仅对阵列特征注释有用，而且对许多研究硬骨鱼渗透调节和毒理学的不同方面的研究小组以及研究各种鳗鱼亚种的种群遗传学和动力学的许多小组都有广泛的影响。

Summary, The evolutionary success of aquatic organisms in many diverse freshwater (FW) and sea water (SW) habitats has been made possible by the development of complex inter-related ion and water transport systems which allow them to osmoregulate and survive in extreme hypotonic and hypertonic environments. Well-studied examples of this are the euryhaline teleosts, such as the European eel, which exhibit the genetic plasticity to enable survival in both FW and SW environments with only minimal changes in the osmolality and ionic composition of their body fluids. The European eel has proved to be an excellent model species for the study of osmoregulation in euryhaline teleosts and recently, work funded by the NERC has enabled the production of EST libraries from the gill, intestine, kidney, gonad and brain which together comprise over 12,000 tissue-specific clones. This eel clone set has been used to produce two separate 18,000 feature microarrays which are produced and currently available for use by the Liverpool Microarray Facility, School of Biological Sciences, The University of Liverpool. However, only less than 1200 of the clones which serve as features on the arrays have been sequenced. This application requests support for the sequencing of the remaining clones and the general housekeeping of the libraries which will involve re-plating all clones to reduce redundancy to below 5%. The re-formulated library will be fully annotated and ESTs characterised in terms of sequence homologies with other teleost genomes (zebrafish, fugu) and to genes deposited within other gene banks. The sequence information will not only be useful for array feature annotation but will also have wide-ranging implications for many research groups working on different aspects of teleost osmoregulation and toxicology as well as the many groups investigating the population genetics and dynamics of various eel sub-species.