MECHANISM AND KINETICS OF RNA FOLDING BY SAXS

SAXS 的 RNA 折叠机制和动力学

• 批准号: 7954894
• 负责人: ROBERT BRIBER
• 金额: $ 5.21万
• 依托单位: ILLINOIS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-01-01 至 2009-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7954894
• 关键词: Area; Azoarcus; Bacteria; Biophysics; Cations; Cells; Charge; Computer Retrieval of Information on Scientific Projects Database; Electrostatics; Funding; Gene Expression Regulation; Grant; Institution; Introns; Kinetics; Microscopic; Pathway interactions; Process; RNA; RNA Folding; RNA Processing; RNA Sequences; RNA replication; Research; Research Personnel; Resources; Roentgen Rays; Role; Source; Specificity; Structure; Tetrahymena; Theoretical model; Thermodynamics; Time; United States National Institutes of Health; Work; group I ribozyme; inorganic phosphate; insight; mutant; protein complex; viral RNA

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Folding of RNA molecules is critical for the functioning of a range of processes in cells, including gene regulation, viral RNA replication, and the assembly of RNA-protein complexes. As part of these processes, RNA must fold into a specific native structure. The folding of RNA starts with the neutralization of negatively charged phosphate groups in RNA by cations. The charge-screened RNA then collapses into compact intermediate state and subsequently folds into unique native structure with tertiary interactions. Characterization of folding pathway is an active area of research in order to understand the microscopic mechanisms of RNA folding. Outstanding questions on the folding process include: (1) details of the kinetics of folding nucleation and collapse, (2) the role of cations and RNA sequence in determining the specificity of collapse, and (3) the detailed distribution of the structural ensemble. In our work, we employ, time-resolved and static small angle X-ray scattering (SAXS) at BioCAT to study kinetics and thermodynamics of structural changes of a group I ribozyme from the bacterium Azoarcus. The work includes both wild type and mutants and compares the findings to the more widely studied Tetrahymena intron. The project will provide insight into the nucleation of RNA tertiary structures and make a comparison with theoretical models of electrostatic interactions in unfolded and folded RNAs.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 RNA分子的折叠对于细胞中一系列过程的功能至关重要， 包括基因调控、病毒RNA复制和RNA-蛋白质组装 配合物作为这些过程的一部分，RNA必须折叠成特定的天然结构。 RNA的折叠始于带负电荷的磷酸基团的中和 RNA中的阳离子。电荷屏蔽的RNA然后折叠成紧凑的中间体 状态，随后折叠成具有三级相互作用的独特天然结构。 折叠途径的表征是一个活跃的研究领域，以了解 RNA折叠的微观机制关于折叠的悬而未决的问题 过程包括：（1）折叠成核和坍塌的动力学细节，（2） 阳离子和RNA序列在确定崩溃的特异性，和（3） 结构总体的详细分布。在我们的工作中，我们雇用，时间解决 和静态小角X射线散射（SAXS）在BioCAT研究动力学和 细菌Ⅰ类核酶结构变化的热力学 Azoarcus这项工作包括野生型和突变型，并将研究结果与 四膜虫内含子的研究更广泛。该项目将提供深入了解 RNA三级结构的成核，并与 未折叠和折叠RNA中的静电相互作用。