MECHANISM AND KINETICS OF RNA FOLDING BY SAXS
MECHANISM AND KINETICS OF RNA FOLDING BY SAXS
批准号:
7601762
负责人:
ROBERT BRIBER
金额:
$2.36万
依托单位国家:
美国
项目类别:
财政年份:
2007
资助国家:
美国
项目状态:
已结题
起止时间:
2007-04-01 至 2008-03-31
关键词:
AddressAzoarcusBacteriaBiological ProcessCationsComplexComputer Retrieval of Information on Scientific Projects DatabaseElectrostaticsEngineeringFundingGene Expression RegulationGrantInstitutionIntronsKineticsLeadPathway interactionsPopulationProteinsRNARNA FoldingRNA SequencesResearchResearch PersonnelResourcesRibosomesRoentgen RaysSourceSpecificityStructureTestingTetrahymenaTheoretical modelUnited States National Institutes of HealthWorkgroup I ribozymeinsightmutantsensortheoriesthree dimensional structuretime use
中文摘要
这个子项目是众多研究子项目之一
英文摘要
This subproject is one of many research subprojects utilizing the
resources provided by a Center grant funded by NIH/NCRR. The subproject and
investigator (PI) may have received primary funding from another NIH source,
and thus could be represented in other CRISP entries. The institution listed is
for the Center, which is not necessarily the institution for the investigator.
RNAs perform many biological functions by folding into specific three-dimensional structures. Understanding how RNAs fold is crucial for understanding the assembly of RNA-protein complexes such as the ribosome, the regulation of gene expression by RNA, and the engineering of RNA sensors and "riboswitches".
In the presence of counterions, RNAs collapse into compact intermediates, which subsequently fold into the native tertiary structure. Theory suggests that competition between different collapse mechanisms partitions the RNA population along alternate folding pathways, some of which lead to kinetically trapped and misfolded intermediates.
Some of the important questions in RNA folding include (1) the kinetic mechanism of nucleation and collapse, (2) how cations and RNA sequence determine the specificity of the initial collapse transition, and (3) the distribution of structures in the ensemble of unfolded RNA. We plan to address these questions using time resolved small angle X-ray scattering (SAXS) on a group I ribozyme from the bacterium Azoarcus. The proposed studies will provide insight into the nucleation of RNA tertiary structure and provide experimental tests of theoretical models of electrostatic interactions in unfolded and folded RNAs. The work will include both wild type and mutants of Azoarcus and comparison to the more widely studied Tetrahymena intron.
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RNA FOLDING STRUCTURE AND KINETICS
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批准号:8361267
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项目类别:
-
资助金额:$6.52万
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财政年份:2011
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负责人:ROBERT BRIBER
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依托单位:
RNA FOLDING STRUCTURE AND KINETICS
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批准号:8168612
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项目类别:
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资助金额:$4.32万
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财政年份:2010
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负责人:ROBERT BRIBER
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依托单位:
MECHANISM AND KINETICS OF RNA FOLDING BY SAXS
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批准号:7954894
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项目类别:
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资助金额:$5.21万
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财政年份:2009
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负责人:ROBERT BRIBER
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依托单位:
MECHANISM AND KINETICS OF RNA FOLDING BY SAXS
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批准号:7722746
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项目类别:
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资助金额:$3.8万
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财政年份:2008
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负责人:ROBERT BRIBER
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依托单位:
COUNTERION DEPENDENT FOLDING OF RNA
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批准号:7369138
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项目类别:
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资助金额:$0.75万
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财政年份:2006
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负责人:ROBERT BRIBER
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依托单位:
COUNTERION DEPENDENT FOLDING OF RNA
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批准号:7182114
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项目类别:
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资助金额:$0.89万
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财政年份:2005
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负责人:ROBERT BRIBER
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依托单位:
FOLDING DYNAMICS OF RNA BY CATION CONDENSATION
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批准号:7182101
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项目类别:
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资助金额:$0.89万
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财政年份:2005
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负责人:ROBERT BRIBER
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依托单位:
FOLDING DYNAMICS OF RNA BY CATION CONDENSATION
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批准号:6975523
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项目类别:
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资助金额:$1.84万
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财政年份:2004
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负责人:ROBERT BRIBER
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依托单位:
国内基金
海外基金
Azoarcus indigens HZ5降解高效氯氰菊酯的代谢途径及机理研究
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批准号:21007058
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项目类别:青年科学基金项目
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资助金额:18.0万元
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批准年份:2010
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负责人:马云
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依托单位: