MECHANISM AND KINETICS OF RNA FOLDING BY SAXS

SAXS 的 RNA 折叠机制和动力学

• 批准号: 7722746
• 负责人: ROBERT BRIBER
• 金额: $ 3.8万
• 依托单位: ILLINOIS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-04-01 至 2008-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7722746
• 关键词: Address; Azoarcus; Bacteria; Biological Process; Cations; Charge; Complex; Computer Retrieval of Information on Scientific Projects Database; Electrostatics; Funding; Gene Expression Regulation; Grant; Institution; Kinetics; Pathway interactions; Population; Proteins; RNA; RNA Folding; RNA Sequences; RNA replication; Research; Research Personnel; Resources; Roentgen Rays; Role; Source; Specificity; Staging; Structure; Testing; Theoretical model; United States National Institutes of Health; Viral; group I ribozyme; inorganic phosphate; insight; time use

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Understanding the folding mechanism of RNA is crucial for understanding biological processes such as gene regulation, viral RNA replication, and the assembly of RNA-protein complexes. Folding of RNAs is driven by neutralization of phosphate charge by cations. In the presence of the cations, RNAs fold into native tertiary structure following an initial collapse into compact intermediates. It is believed that different folding pathways partition the RNA population, some of which is kinetically trapped in misfolded intermediates. However, the details of the RNA folding mechanisms remain unclear, including the following important questions: (1) the kinetic mechanism of folding nucleation and collapse, (2) the role of cations and RNA sequence in determining specificity of collapse, and (3) the breadth of the structural ensemble. In order to address these questions, we are using time-resolved and static small angle X-ray scattering (SAXS) at BioCAT on a group I ribozyme from the bacterium Azoarcus. The results will give insight into the early stages of RNA folding and provide experimental tests of theoretical models of electrostatic interactions in unfolded and folded RNAs.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 了解 RNA 的折叠机制对于理解基因调控、病毒 RNA 复制和 RNA-蛋白质复合物组装等生物过程至关重要。 RNA 的折叠是由阳离子中和磷酸盐电荷驱动的。在存在阳离子的情况下，RNA 在最初折叠成紧凑的中间体后折叠成天然三级结构。据信，不同的折叠途径将RNA群体分开，其中一些在动力学上被困在错误折叠的中间体中。然而，RNA折叠机制的细节仍不清楚，包括以下重要问题：（1）折叠成核和塌陷的动力学机制，（2）阳离子和RNA序列在确定塌陷特异性中的作用，以及（3）结构整体的广度。为了解决这些问题，我们在 BioCAT 对来自 Azoarcus 细菌的 I 组核酶使用时间分辨静态小角 X 射线散射 (SAXS)。这些结果将深入了解 RNA 折叠的早期阶段，并为未折叠和折叠 RNA 中静电相互作用的理论模型提供实验测试。