MPHR(A): TRANSCRIPTION FACTOR REQUIRED FOR ERYTHROMYCIN RESISTANCE
MPHR(A): TRANSCRIPTION FACTOR REQUIRED FOR ERYTHROMYCIN RESISTANCE
批准号:
7955174
负责人:
Nicole A LaRonde
金额:
$0.39万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2009
资助国家:
美国
项目状态:
已结题
起止时间:
2009-04-01 至 2010-03-31
关键词:
BindingBiologicalComputer Retrieval of Information on Scientific Projects DatabaseDNAEngineeringErythromycinFundingGene ExpressionGenesGoalsGrantInitiator CodonInstitutionMacrolide AntibioticsMacrolidesPhosphotransferasesProteinsResearchResearch PersonnelResistanceResourcesSourceStructureUnited States National Institutes of Healthgenetic regulatory proteinstructural biologytranscription factor
中文摘要
这个子项目是许多研究子项目中利用
资源由NIH/NCRR资助的中心拨款提供。子项目和
调查员(PI)可能从NIH的另一个来源获得了主要资金,
并因此可以在其他清晰的条目中表示。列出的机构是
该中心不一定是调查人员的机构。
调节蛋白MPHR(A)最近被广泛应用于合成生物学应用,如代谢物传感和外源基因表达控制。该蛋白通过与起始密码子上游35bp的DNA操纵子结合,负调控大环内酯类2‘-磷酸转移酶I抗性基因(MphA)的表达,并被红霉素抑制。我们在这个项目中的目标是确定红霉素结合的MPHR(A)和游离的MPHR(A)在结构上的差异,以便了解它是如何被改造成对不同的大环内酯类抗生素反应的。
英文摘要
This subproject is one of many research subprojects utilizing the
resources provided by a Center grant funded by NIH/NCRR. The subproject and
investigator (PI) may have received primary funding from another NIH source,
and thus could be represented in other CRISP entries. The institution listed is
for the Center, which is not necessarily the institution for the investigator.
The regulatory protein MphR(A) has recently seen extensive use in Synthetic Biological applications such as metabolite sensing and exogenous control of gene expression. This protein negatively regulates the expression of a macrolide 2'-phosphotransferase I resistance gene (mphA) via binding to a 35bp DNA operator upstream of the start codon, and is de-repressed by the presence of erythromycin. Our goal in this project was to determine the difference in structure between the erythromycin bound and free Mphr(A) in order to understand how it might be engineered to respond to different macrolide antibiotics.
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STRUCTURAL STUDIES OF VARIOUS MACROMOLECULES
-
批准号:8361665
-
项目类别:
-
资助金额:$2.74万
-
财政年份:2011
-
负责人:Nicole A LaRonde
-
依托单位:
STRUCTURAL STUDIES OF VARIOUS MACROMOLECULES
-
批准号:8169316
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项目类别:
-
资助金额:$0.7万
-
财政年份:2010
-
负责人:Nicole A LaRonde
-
依托单位:
RIBOSOME BIOGENESIS
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批准号:7955171
-
项目类别:
-
资助金额:$0.39万
-
财政年份:2009
-
负责人:Nicole A LaRonde
-
依托单位:
IODOTYROSINE DEHALOGENASE, AN ENZYME REQUIRED FOR IODINE SALVAGE
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批准号:7955175
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项目类别:
-
资助金额:$0.39万
-
财政年份:2009
-
负责人:Nicole A LaRonde
-
依托单位:
GLUTAMINE DEPENDENT NAD+ SYNTHETASE FROM MYCOBACTERIUM TUBERCULOSIS
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批准号:7955172
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项目类别:
-
资助金额:$0.39万
-
财政年份:2009
-
负责人:Nicole A LaRonde
-
依托单位:
STRUCTURAL STUDIES OF THE MYCOBACTERIUM ESX-1 SECRETION SYSTEM
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批准号:7955173
-
项目类别:
-
资助金额:$0.39万
-
财政年份:2009
-
负责人:Nicole A LaRonde
-
依托单位:
Structural and functional studies of the human RIO kinases
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批准号:7264828
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项目类别:
-
资助金额:$16.35万
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财政年份:2007
-
负责人:Nicole A LaRonde
-
依托单位:
Structural and functional studies of the human RIO kinases
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批准号:7487503
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项目类别:
-
资助金额:$16.42万
-
财政年份:2007
-
负责人:Nicole A LaRonde
-
依托单位:
Structural and functional studies of the human RIO kinases
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批准号:7658825
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项目类别:
-
资助金额:$16.42万
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财政年份:2007
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负责人:Nicole A LaRonde
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依托单位:
海外基金