MPHR(A): TRANSCRIPTION FACTOR REQUIRED FOR ERYTHROMYCIN RESISTANCE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 调节蛋白MPHR(A)最近被广泛应用于合成生物学应用，如代谢物传感和外源基因表达控制。该蛋白通过与起始密码子上游35bp的DNA操纵子结合，负调控大环内酯类2‘-磷酸转移酶I抗性基因(MphA)的表达，并被红霉素抑制。我们在这个项目中的目标是确定红霉素结合的MPHR(A)和游离的MPHR(A)在结构上的差异，以便了解它是如何被改造成对不同的大环内酯类抗生素反应的。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The regulatory protein MphR(A) has recently seen extensive use in Synthetic Biological applications such as metabolite sensing and exogenous control of gene expression. This protein negatively regulates the expression of a macrolide 2'-phosphotransferase I resistance gene (mphA) via binding to a 35bp DNA operator upstream of the start codon, and is de-repressed by the presence of erythromycin. Our goal in this project was to determine the difference in structure between the erythromycin bound and free Mphr(A) in order to understand how it might be engineered to respond to different macrolide antibiotics.