MPHR(A): TRANSCRIPTION FACTOR REQUIRED FOR ERYTHROMYCIN RESISTANCE
MPHR(A): TRANSCRIPTION FACTOR REQUIRED FOR ERYTHROMYCIN RESISTANCE
批准号:
7955174
负责人:
Nicole A LaRonde
金额:
$0.39万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2009
资助国家:
美国
项目状态:
已结题
起止时间:
2009-04-01 至 2010-03-31
关键词:
BindingBiologicalComputer Retrieval of Information on Scientific Projects DatabaseDNAEngineeringErythromycinFundingGene ExpressionGenesGoalsGrantInitiator CodonInstitutionMacrolide AntibioticsMacrolidesPhosphotransferasesProteinsResearchResearch PersonnelResistanceResourcesSourceStructureUnited States National Institutes of Healthgenetic regulatory proteinstructural biologytranscription factor
中文摘要
这个子项目是众多研究子项目之一
英文摘要
This subproject is one of many research subprojects utilizing the
resources provided by a Center grant funded by NIH/NCRR. The subproject and
investigator (PI) may have received primary funding from another NIH source,
and thus could be represented in other CRISP entries. The institution listed is
for the Center, which is not necessarily the institution for the investigator.
The regulatory protein MphR(A) has recently seen extensive use in Synthetic Biological applications such as metabolite sensing and exogenous control of gene expression. This protein negatively regulates the expression of a macrolide 2'-phosphotransferase I resistance gene (mphA) via binding to a 35bp DNA operator upstream of the start codon, and is de-repressed by the presence of erythromycin. Our goal in this project was to determine the difference in structure between the erythromycin bound and free Mphr(A) in order to understand how it might be engineered to respond to different macrolide antibiotics.
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会议论文
STRUCTURAL STUDIES OF VARIOUS MACROMOLECULES
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批准号:8361665
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项目类别:
-
资助金额:$2.74万
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财政年份:2011
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负责人:Nicole A LaRonde
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依托单位:
STRUCTURAL STUDIES OF VARIOUS MACROMOLECULES
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批准号:8169316
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项目类别:
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资助金额:$0.7万
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财政年份:2010
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负责人:Nicole A LaRonde
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依托单位:
RIBOSOME BIOGENESIS
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批准号:7955171
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项目类别:
-
资助金额:$0.39万
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财政年份:2009
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负责人:Nicole A LaRonde
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依托单位:
IODOTYROSINE DEHALOGENASE, AN ENZYME REQUIRED FOR IODINE SALVAGE
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批准号:7955175
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项目类别:
-
资助金额:$0.39万
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财政年份:2009
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负责人:Nicole A LaRonde
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依托单位:
GLUTAMINE DEPENDENT NAD+ SYNTHETASE FROM MYCOBACTERIUM TUBERCULOSIS
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批准号:7955172
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项目类别:
-
资助金额:$0.39万
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财政年份:2009
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负责人:Nicole A LaRonde
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依托单位:
STRUCTURAL STUDIES OF THE MYCOBACTERIUM ESX-1 SECRETION SYSTEM
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批准号:7955173
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项目类别:
-
资助金额:$0.39万
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财政年份:2009
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负责人:Nicole A LaRonde
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依托单位:
Structural and functional studies of the human RIO kinases
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批准号:7264828
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项目类别:
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资助金额:$16.35万
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财政年份:2007
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负责人:Nicole A LaRonde
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依托单位:
Structural and functional studies of the human RIO kinases
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批准号:7487503
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项目类别:
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资助金额:$16.42万
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财政年份:2007
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负责人:Nicole A LaRonde
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依托单位:
Structural and functional studies of the human RIO kinases
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批准号:7658825
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项目类别:
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资助金额:$16.42万
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财政年份:2007
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负责人:Nicole A LaRonde
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依托单位:
海外基金