THE UBIQUITIN PROTEASOME SYSTEM (UPS)

泛素蛋白酶体系统 (UPS)

• 批准号: 7955177
• 负责人: FRANK SICHERI
• 金额: $ 1.61万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-01 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7955177
• 关键词: 26S proteasome; Cell Cycle; Cell Cycle Progression; Computer Retrieval of Information on Scientific Projects Database; Enzymes; Funding; Grant; Institution; Phosphorylation; Polyubiquitin; Proteins; Research; Research Personnel; Resources; Source; System; Ubiquitin; Ubiquitin-Activating Enzymes; Ubiquitin-Conjugating Enzymes; United States National Institutes of Health; interest; multicatalytic endopeptidase complex; structural biology; ubiquitin ligase

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The ubiquitin proteasome system (UPS) is made up of a sequentially acting set of enzymes termed E1(ubiquitin activating enzyme), E2 (ubiquitin conjugating enzyme), and E3s (ubiquitin ligase). The action of the UPS cascade is responsible for the elimination of ubiquitinated proteins including many that regulate cell cycle progression. The phosphorylation of cell cycle substrates allows for their recognition by a subset of E3 enzymes. Polyubiquitin chains are then added to the substrate which targets them to the 26S proteasome for degradation. In my lab we are interested in understanding the catalytic mechanism of ubiquitin transfer, the mechanism by which UPS components are regulated and the mechanism by which E3 enzymes recognize their substrates.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 泛素蛋白酶体系统（UPS）由称为E1（泛素活化酶）、E2（泛素缀合酶）和E3 s（泛素连接酶）的顺序作用的酶组组成。 UPS级联的作用是负责消除泛素化蛋白，包括许多调节细胞周期进程的蛋白。 细胞周期底物的磷酸化允许它们被E3酶的子集识别。 然后将多聚泛素链添加到底物中，底物将其靶向26 S蛋白酶体进行降解。 在我的实验室，我们有兴趣了解泛素转移的催化机制，UPS组件的调节机制和E3酶识别其底物的机制。