HUMAN PLASMA PLATELET ACTIVATING FACTOR ACETYLHYDROLASE

人血浆血小板激活因子乙酰水解酶

• 批准号: 7955196
• 负责人: UTTAMKUMAR SAMANTA
• 金额: $ 0.43万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-01 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7955196
• 关键词: Allergic Reaction; Anaphylaxis; Anti-Inflammatory Agents; Anti-inflammatory; Asthma; Binding; Catalysis; Code; Complex; Computer Retrieval of Information on Scientific Projects Database; Data; Enzymes; Funding; Grant; Homologous Gene; Human; Hydrolysis; Institution; Link; Low-Density Lipoproteins; Membrane Proteins; Organophosphates; Plasma; Platelet Activating Factor; Publishing; Research; Research Personnel; Resources; Sarin; Soman; Source; Structure; United States National Institutes of Health; structural biology

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The plasma form of human platelet activating factor acetylhydrolase (pPAFAH) functions by reducing PAF levels as a general anti-inflammatory scavenger and is linked to anaphylactic shock, asthma and allergic reactions. As a membrane associated protein with no known homologues, pPAFAH is a worthy structural target. Recently we have published the crystal structure of this important enzyme and shown how enzyme binds to LDL interface during catalysis. Data collected from 24ID would be used to solve the crystal structures of pPAFAH complex with sarin and soman and useful to predict the mode of substrate binding to the enzyme and hydrolysis of toxic organophosphates. PDB codes for the complex structures for which data collected at 24ID are 3F96 and 3F97 for sarin and soman, respectively.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 血浆形式的人血小板活化因子乙酰水解酶（pPAFAH）通过降低PAF水平作为一般抗炎清除剂起作用，并且与过敏性休克、哮喘和过敏反应有关。pPAFAH是一种膜相关蛋白，没有已知的同源物，是一个有价值的结构靶标。最近我们发表了这种重要酶的晶体结构，并显示了酶在催化过程中如何与LDL界面结合。从24 ID收集的数据将被用来解决pPAFAH与沙林和梭曼复合物的晶体结构和有用的预测底物结合的酶和有毒有机磷酸酯的水解的模式。 在24 ID处收集的数据为3F 96的复杂结构的PDB代码， 沙林和梭曼分别为3F 97。