UDE COBRE: PEPTIDE-BASED BIOMATERIALS WITH ENVIRON SENSITIVE MORPHOLOGIES

UDE COBRE：具有环境敏感形态的肽基生物材料

• 批准号: 7960410
• 负责人: JOEL P SCHNEIDER
• 金额: $ 40.18万
• 依托单位: UNIVERSITY OF DELAWARE
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-01 至 2010-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7960410
• 关键词: Amino Acids; Biocompatible; Biocompatible Materials; Biotechnology; Cell Adhesion; Centers of Research Excellence; Chemistry; Computer Retrieval of Information on Scientific Projects Database; Cues; Diffusion; Fibroblasts; Funding; Grant; Hydrogels; Institution; Ionic Strengths; Light; Microfluidics; Molecular; Molecular Conformation; Molecular Weight; Morphology; Mus; Nature; Nutrient; Peptides; Polymers; Property; Prunella vulgaris; Research; Research Personnel; Resources; Source; System; Temperature; Tissue Engineering; United States National Institutes of Health; base; cell motility; crosslink; design; nanoscale; novel; porous hydrogel; response; scaffold; self assembly; wasting

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Project II: Schneider and Pochan Hydrogels, a class of materials integral to biotechnologies such as tissue engineering and microfluidics are classically made from high molecular weight polymers. Ideal hydrogel scaffolds for use in tissue engineering are easily synthesized using non-toxic chemistries, biocompatible, promote cell adhesion/proliferation and are biodegradable. In terms of material properties, their morphology should be porous for cell motility and nutrient/waste diffusion. However, despite their porous nature, these materials must be mechanically rigid. We developed an alternate strategy that employs self-assembling peptides to prepare responsive hydrogels. Preparing materials from peptides is advantageous since peptides are quickly synthesized and novel amino acid residues readily incorporated for tailored functions. Also, since our hydrogels are prepared from a pure self-assembly mechanism, there is no need for exogenous toxic cross linking agents. The strategy employs designed peptides that intramolecularly fold on cue into a b-hairpin conformation that is amenable to self-assembly. Key to this system is that the peptide must be intramolecularly folded before self-assembly leading to hydrogelation can occur. By designing peptides that only fold when desired environmental cues are present, responsive materials may be constructed. Hairpins have been designed to fold in response to pH and ionic strength changes, light, and temperature changes. Hydrogels are porous and well hydrated on the micro- and nano-scale, but are rigid (G'=1600Pa), shear thin and self heals after cessation of shear. Importantly, these hydrogels support the attachment and proliferation of NIH 3T3 fibroblasts (mouse), indicating their possible use as tissue engineering scaffolds.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 项目二：施耐德和Pochan 水凝胶是生物技术（如组织工程和微流体）不可或缺的一类材料，通常由高分子量聚合物制成。 用于组织工程的理想水凝胶支架易于使用无毒化学物质合成，具有生物相容性，促进细胞粘附/增殖，并且是可生物降解的。 就材料性质而言，它们的形态应该是多孔的，以便于细胞运动和营养物/废物扩散。 然而，尽管它们具有多孔性质，但这些材料必须是机械刚性的。 我们开发了一种替代策略，采用自组装肽来制备响应性水凝胶。 从肽制备材料是有利的，因为肽快速合成并且新的氨基酸残基容易并入以用于定制的功能。 此外，由于我们的水凝胶是由纯自组装机制制备的，因此不需要外源毒性交联剂。 该策略采用设计的肽，分子内折叠成b-发夹构象，这是服从自组装的线索。 这个系统的关键是，肽必须在分子内折叠，然后才能发生自组装，导致水凝胶化。 通过设计仅在所需环境线索存在时折叠的肽，可以构建响应性材料。 发夹被设计成响应于pH和离子强度变化、光和温度变化而折叠。 水凝胶是多孔的并且在微米和纳米尺度上充分水合，但是是刚性的（G '= 1600 Pa），剪切稀的并且在剪切停止后自愈合。 重要的是，这些水凝胶支持NIH 3 T3成纤维细胞（小鼠）的附着和增殖，表明它们可能用作组织工程支架。