KY COBRE: PROTEOMICS CORE

KY COBRE:蛋白质组学核心

基本信息

  • 批准号:
    7960494
  • 负责人:
  • 金额:
    $ 8.23万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2009
  • 资助国家:
    美国
  • 起止时间:
    2009-09-01 至 2010-06-30
  • 项目状态:
    已结题

项目摘要

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The proteomics core is designed to provide quantitative and qualitative protein profiling and identification analysis, as well as post-translational modification and sequence analysis through a combination of 2D gel, mass spectrometry and Edman sequencing analysis services. The core is equipped with Bio-Rad IPG and SDS-PAGE apparatus for 2D gel analysis, a Typhoon phosphorimager/scanner system for detection and quantitation of fluorescent protein dyes (Cy-dyes) and stains (SYPRO-Ruby, Pro-Q Diamond) used to analyze 1D and 2D gels, a Ciphergen SELDI-TOF protein profiling system, an ABI QSTAR II Q-TOF Mass Spectrometer with both MALDI and LC/ESI source capabilities, Waters HPLC systems and multiple PCs for data acquisition and analysis including a site license for PDQuest software and a 5 station site license for Ciphergen software. Fully trained technical staff will assist investigators with the full range of proteomics analytical services. The staff will instruct investigators and their laboratory staff (students, post-doctoral fellows and technicians) in sample preparation, 2D gel analysis and quantitation by fluorescent staining methods. Protein fragmentation, mass peptide mapping and protein sequencing by both MS/MS mass spectrometry and chemical sequencing will be performed by Dr. Beach on samples provided by individual investigators. Preparation and analysis of samples with the Ciphergen system will be facilitated by automated robotic work stations. Data analysis will be performed by investigators and their colleagues through multiple distant work stations connected to the Ciphergen system via the web.
这个子项目是众多研究子项目之一

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

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Haining Zhu其他文献

Haining Zhu的其他文献

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{{ truncateString('Haining Zhu', 18)}}的其他基金

BLRD Research Career Scientist Award Application
BLRD 研究职业科学家奖申请
  • 批准号:
    10703154
  • 财政年份:
    2023
  • 资助金额:
    $ 8.23万
  • 项目类别:
RNA Surveillance and Protein Translation in FTD
FTD 中的 RNA 监测和蛋白质翻译
  • 批准号:
    10687846
  • 财政年份:
    2021
  • 资助金额:
    $ 8.23万
  • 项目类别:
RNA Surveillance and Protein Translation in FTD
FTD 中的 RNA 监测和蛋白质翻译
  • 批准号:
    10449486
  • 财政年份:
    2021
  • 资助金额:
    $ 8.23万
  • 项目类别:
RNA Surveillance and Protein Translation in FTD
FTD 中的 RNA 监测和蛋白质翻译
  • 批准号:
    10455737
  • 财政年份:
    2021
  • 资助金额:
    $ 8.23万
  • 项目类别:
FUS Protein Homeostasis in ALS
ALS 中的 FUS 蛋白稳态
  • 批准号:
    9892565
  • 财政年份:
    2015
  • 资助金额:
    $ 8.23万
  • 项目类别:
FUS Protein Homeostasis in ALS
ALS 中的 FUS 蛋白稳态
  • 批准号:
    10620292
  • 财政年份:
    2015
  • 资助金额:
    $ 8.23万
  • 项目类别:
FUS Protein Homeostasis in ALS
ALS 中的 FUS 蛋白稳态
  • 批准号:
    10550115
  • 财政年份:
    2015
  • 资助金额:
    $ 8.23万
  • 项目类别:
Role of FUS in ALS
FUS 在 ALS 中的作用
  • 批准号:
    8234739
  • 财政年份:
    2011
  • 资助金额:
    $ 8.23万
  • 项目类别:
Role of FUS in ALS
FUS 在 ALS 中的作用
  • 批准号:
    8313863
  • 财政年份:
    2011
  • 资助金额:
    $ 8.23万
  • 项目类别:
PROTEOMICS CORE
蛋白质组学核心
  • 批准号:
    8360573
  • 财政年份:
    2011
  • 资助金额:
    $ 8.23万
  • 项目类别:
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