PROTEOMICS CORE

蛋白质组学核心

• 批准号: 8360573
• 负责人: Haining Zhu
• 金额: $ 9.56万
• 依托单位: UNIVERSITY OF KENTUCKY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8360573
• 关键词: Amino Acid Sequence; Centers of Research Excellence; Chemicals; Computer software; Data Analyses; Detection; Diamond; Distant; Dyes; Funding; Gel; Grant; High Pressure Liquid Chromatography; Individual; Internet; Laboratories; Licensing; Mass Spectrum Analysis; Molecular; National Center for Research Resources; Peptide Mapping; Peptide Sequence Determination; Post-Translational Protein Processing; Postdoctoral Fellow; Preparation; Principal Investigator; Pro-Q aerosol foam; Proteins; Proteomics; Research; Research Infrastructure; Research Personnel; Resources; Robotics; Sampling; Sequence Analysis; Services; Site; Source; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Staining method; Stains; Students; System; Training; United States National Institutes of Health; Water; Work; aerobic respiration control protein; base; cost; data acquisition; design; human disease; mass spectrometer; protein profiling

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The proteomics core is designed to provide quantitative and qualitative protein profiling and identification analysis, as well as post-translational modification and sequence analysis through a combination of 2D gel, mass spectrometry and Edman sequencing analysis services. The core is equipped with Bio-Rad IPG and SDS-PAGE apparatus for 2D gel analysis, a Typhoon phosphorimager/scanner system for detection and quantitation of fluorescent protein dyes (Cy-dyes) and stains (SYPRO-Ruby, Pro-Q Diamond) used to analyze 1D and 2D gels, a Ciphergen SELDI-TOF protein profiling system, an ABI QSTAR II Q-TOF Mass Spectrometer with both MALDI and LC/ESI source capabilities, Waters HPLC systems and multiple PCs for data acquisition and analysis including a site license for PDQuest software and a 5 station site license for Ciphergen software. Fully trained technical staff will assist investigators with the full range of proteomics analytical services. The staff will instruct investigators and their laboratory staff (students, post-doctoral fellows and technicians) in sample preparation, 2D gel analysis and quantitation by fluorescent staining methods. Protein fragmentation, mass peptide mapping and protein sequencing by both MS/MS mass spectrometry and chemical sequencing will be performed by Dr. Beach on samples provided by individual investigators. Preparation and analysis of samples with the Ciphergen system will be facilitated by automated robotic work stations. Data analysis will be performed by investigators and their colleagues through multiple distant work stations connected to the Ciphergen system via the web.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 蛋白质组学核心旨在提供定量和定性蛋白质分析和鉴定分析，以及通过2D凝胶，质谱和Edman测序分析服务的组合进行翻译后修饰和序列分析。核心配备了用于2D凝胶分析的Bio-Rad IPG和SDS-PAGE装置，用于检测和定量荧光蛋白染料（Cy-dyes）和染色剂的Typhoon磷光成像仪/扫描仪系统（SYPRO-Ruby，Pro-Q Diamond），Ciphergen SELDI-TOF蛋白质谱分析系统，具有MALDI和LC/ESI源功能的ABI QSTAR II Q-TOF质谱仪，用于数据采集和分析的沃茨HPLC系统和多台PC，包括PDQuest软件的现场许可证和Ciphergen软件的5站现场许可证。训练有素的技术人员将协助研究人员提供全方位的蛋白质组学分析服务。工作人员将指导研究人员及其实验室工作人员（学生，博士后研究员和技术人员）进行样品制备，2D凝胶分析和荧光染色方法定量。由Dr.海滩对个体研究者提供的样本进行蛋白质片段化、质量肽图谱分析以及通过MS/MS质谱法和化学测序法进行蛋白质测序。自动化机器人工作站将有助于用Ciphergen系统制备和分析样品。数据分析将由研究者及其同事通过网络连接到Ciphergen系统的多个远程工作站进行。