PROTEOMICS CORE

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 蛋白质组学核心旨在提供定量和定性蛋白质分析和鉴定分析，以及通过2D凝胶，质谱和Edman测序分析服务的组合进行翻译后修饰和序列分析。核心配备了用于2D凝胶分析的Bio-Rad IPG和SDS-PAGE装置，用于检测和定量荧光蛋白染料（Cy-dyes）和染色剂的Typhoon磷光成像仪/扫描仪系统（SYPRO-Ruby，Pro-Q Diamond），Ciphergen SELDI-TOF蛋白质谱分析系统，具有MALDI和LC/ESI源功能的ABI QSTAR II Q-TOF质谱仪，用于数据采集和分析的沃茨HPLC系统和多台PC，包括PDQuest软件的现场许可证和Ciphergen软件的5站现场许可证。训练有素的技术人员将协助研究人员提供全方位的蛋白质组学分析服务。工作人员将指导研究人员及其实验室 在样品制备、2D凝胶分析和荧光染色定量方法方面，由Dr.海滩对个体研究者提供的样本进行蛋白质片段化、质量肽图谱分析以及通过MS/MS质谱法和化学测序法进行蛋白质测序。自动化机器人工作站将有助于用Ciphergen系统制备和分析样品。数据分析将由研究者及其同事通过网络连接到Ciphergen系统的多个远程工作站进行。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The proteomics core is designed to provide quantitative and qualitative protein profiling and identification analysis, as well as post-translational modification and sequence analysis through a combination of 2D gel, mass spectrometry and Edman sequencing analysis services. The core is equipped with Bio-Rad IPG and SDS-PAGE apparatus for 2D gel analysis, a Typhoon phosphorimager/scanner system for detection and quantitation of fluorescent protein dyes (Cy-dyes) and stains (SYPRO-Ruby, Pro-Q Diamond) used to analyze 1D and 2D gels, a Ciphergen SELDI-TOF protein profiling system, an ABI QSTAR II Q-TOF Mass Spectrometer with both MALDI and LC/ESI source capabilities, Waters HPLC systems and multiple PCs for data acquisition and analysis including a site license for PDQuest software and a 5 station site license for Ciphergen software. Fully trained technical staff will assist investigators with the full range of proteomics analytical services. The staff will instruct investigators and their laboratory staff (students, post-doctoral fellows and technicians) in sample preparation, 2D gel analysis and quantitation by fluorescent staining methods. Protein fragmentation, mass peptide mapping and protein sequencing by both MS/MS mass spectrometry and chemical sequencing will be performed by Dr. Beach on samples provided by individual investigators. Preparation and analysis of samples with the Ciphergen system will be facilitated by automated robotic work stations. Data analysis will be performed by investigators and their colleagues through multiple distant work stations connected to the Ciphergen system via the web.