EVOLUTIONARY CONSEQUENCES OF DENGUE VIRUS EMERGENCE

登革热病毒出现的进化后果

• 批准号: 7960227
• 负责人: Kathryn Alyce Hanley
• 金额: $ 6.44万
• 依托单位: NEW MEXICO STATE UNIVERSITY LAS CRUCES
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-01 至 2010-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7960227
• 关键词: Aedes; Africa; Arboviruses; Biomedical Research; Computer Retrieval of Information on Scientific Projects Database; Culicidae; Dengue; Dengue Virus; Disease Outbreaks; Funding; Future; Genetic; Grant; Human; Infection; Institution; Life Cycle Stages; New Mexico; Outcome; Public Health; Research; Research Personnel; Resources; Serotyping; Source; Southeastern Asia; Testing; Time; United States National Institutes of Health; Vaccines; Virus; forest; nonhuman primate; prevent; vector

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Mosquito-transmitted dengue virus, the agent of dengue fever, poses the most significant threat to public health of any arthropod-borne virus. Dengue viruses circulate in two distinct life cycles: (i) a global endemic cycle between humans and peridomestic Aedes mosquitoes, and (ii) a sylvatic cycle in the forests of West Africa and southeast Asia between arboreal Aedes mosquitoes and non-human primates. Endemic and sylvatic viruses show high genetic similarity, but sylvatic viruses do not cause outbreaks in humans. Our research has focused on identifying the barriers that limit the emergence of sylvatic dengue viruses. This is particularly important because if an effective dengue vaccine were developed, such barriers could come down. The current study tested the hypothesis that competition from established endemic strains could prevent sylvatic viruses from infecting peridomestic vector. We infected groups of Aedes aegypti, the major peridomestic vector, with one of 6 target virus strains: 3 strains of endemic dengue (serotype 2) and 3 strains of sylvatic dengue (serotype 2). Moreover each target strain was used in one of two treatments: single infection or mixed, concurrent infection with a common competitor (dengue serotype 4). We found that replication of each of the six target strains was unaffected by competition, since target virus concentration in the single-strain infections was not different from that of the mixed-strain infections. Future research will focus on the outcome of competition when the introduction of competitors is staggered in time.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 登革热剂的蚊子传播登革热病毒对任何节肢动物传播病毒构成了最大的公共卫生威胁。登革热病毒在两个不同的生命周期中循环：（i）人类和植物性伊蚊之间的全球流行周期，以及（ii）西非森林和植物埃德斯亚森林森林中的sylvatic循环。 流行和肤色病毒显示出很高的遗传相似性，但是sylvatic病毒不会引起人类暴发。我们的研究重点是确定限制登记登革热病毒出现的障碍。 这尤其重要，因为如果开发了有效的登革热疫苗，则可能会降低这种障碍。当前的研究检验了以下假设：既定的地方性菌株的竞争可以防止Sylvatic病毒感染植物植物的载体。我们感染了主要的植物植物载体的埃及埃及埃及的组，其中有6种靶病毒菌株之一：3种特有登革热菌株（血清型2）和3种sylvatic登革热菌株（血清型2）。 此外，每种靶标菌株都用于两种治疗方法之一：与普通竞争者的单一感染或混合感染（登革热血清型4）。 我们发现，六个目标菌株中每一个的复制不受竞争的影响，因为单元感染中的靶病毒浓度与混合应变感染没有差异。当竞争对手的引入及时地引入时，未来的研究将重点放在竞争的结果上。