Coordinating Research on Emerging Arboviral Threats Encompassing the Neotropics (CREATE-NEO)

摘要 新热带地区新出现虫媒病毒威胁的协调研究（CREATE-NEO） 该网络在中美洲和南美洲提供了一个灵活灵活的监测站点网络， 预测和应对新出现的人畜共患病和虫媒病毒的尖端建模方法。创建- NEO预警地方、区域和全球公共卫生机构的人畜共患病和虫媒病毒， 特别是高风险的溢出，出现人与人之间的传播，和/或国际传播。的 母公司拨款1U 01 AI 151807支持在出现人畜共患病的典型条件下开展CREATE-NEO行动 和虫媒病毒活性。自2020年以来，我们已经建立了检测，预测和应对此类突发事件的本地能力 在事件的起源点，从而最大限度地避免全面爆发的可能性。关键活动 包括：（i）登革热的基因组监测，包括深度测序和全面的系统发育分析 巴西圣何塞杜里奥普雷托（SJdRP）的病媒和非人灵长类动物监测， 巴拿马;以及（iii）与下列疾病相关的寨卡、登革热和伊尔赫斯病毒感染人间病例的特征 脑血管和脑炎事件。随着COVID-19疫情的爆发，我们扩大了业务范围， 包括：（iv）SARS-CoV-2和登革热病毒合并感染人间病例的特征;（v）监测 （vi）支持对SARS-CoV-2变异体的基因组监测， SJdRP和巴拿马;及（vii）支持在巴拿马和巴西进行虫媒病毒传播的临床监测。 因此，SARS-CoV-2的出现和迅速传播提供了一个例子，说明我们有能力迅速改变方向， 资源，以应对任何新出现的动物传染病或病媒传播疾病。U 01补充将继续， 扩大这些紧急活动，以支持NIH资助的和其他国际SARS-CoV-2研究 通过以下与母基金最初目标有关的补充活动。我们将扩大我们 SJdRP和巴拿马的SARS-CoV-2变异体的基因组监测能力。我们将描述这两个 通过使用Illumina技术进行深度测序， 使用SARS-COV-2的完整基因组序列进行全面的系统发育研究， 病毒传播，并告知关于传播性和临床结果变化的假设（目标2 e）。

ABSTRACT The Coordinating Research on Emerging Arboviral Threats Encompassing the Neotropics (CREATE-NEO) network provides a nimble and flexible network of surveillance sites in Central and South America coupled to cutting-edge modeling approaches to anticipate and counter emerging zoonoses and arboviruses. CREATE- NEO forewarns local, regional, and global public health agencies of zoonoses and arboviruses that pose particularly high risk of spillover, emergence into transmission among humans, and/or international spread. The parent grant 1U01 AI151807 supports CREATE-NEO operations during typical conditions of emerging zoonoses and arbovirus activity. Since, 2020 we have built local capacity to detect, predict and respond to such emergence events at their point of origin, thereby maximizing the potential to avert full-blown emergence. Critical activities included: (i) genomic surveillance with deep sequencing and comprehensive phylogenetic analyses of dengue virus in Sao Jose do Rio Preto (SJdRP), Brazil; (ii) vector and non-human primate surveillance in Brazil and Panama; and (iii) characterization of human cases of Zika, dengue and Ilheus virus infections associated with cerebrovascular and encephalitic events. With the onset of the COVID-19 pandemic, we expanded our activities to include: (iv) characterization of human cases of SARS-CoV-2 and dengue virus coinfection; (v) surveillance of NHPs for potential spillback of SARS-CoV-2; (vi) supporting genomic surveillance of SARS-CoV-2 variants in SJdRP and Panama; and (vii) supporting clinical surveillance of arbovirus circulation in Panama and Brazil. Thus, the emergence and rapid spread of SARs-CoV-2 offers an example of our capacity to quickly redirect resources to address any emerging zoonotic or vector-borne diseases. This U01 supplement will continue and expand these emergency activities in support of NIH-funded and other international research on SARS-CoV-2 through the following supplemental activities related to the parent grant’s original aims. We will expand our genomic surveillance capabilities of SARS-CoV-2 variants in SJdRP and Panama. We will characterize both between-host and within-host diversity through deep sequencing using Illumina technologies and perform comprehensive phylogenetic studies using complete genomic sequences of SARS-COV-2 to infer patterns of virus spread and to inform hypotheses regarding variation in transmissibility and clinical outcomes (Aim 2e).